NDLI: USP11 Is a Negative Regulator to γH2AX Ubiquitylation by RNF8/RNF168

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USP11 Is a Negative Regulator to γH2AX Ubiquitylation by RNF8/RNF168

Content Provider	Scilit
Author	Yu, Miao Liu, Kun Mao, Zebin Luo, Jianyuan Gu, Wei Zhao, Wenhui
Copyright Year	2016
Description	Journal: Journal of Biological Chemistry Ubiquitin modification at double strand breaks (DSB) sites is an essential regulator of signaling and repair. γH2AX extends from DSB sites and provides a platform for subsequent recruitment and amplification of DNA repair proteins and signaling factors. Here, we found that RNF8/RNF168 ubiquitylates γH2AX. We identified that USP11 is a unique deubiquitylation enzyme for γH2AX. USP11 deubiquitylates γH2AX both in vivo and in vitro but not the canonical (ub)-K119-H2A and (ub)-K120-H2B in vitro, and USP11 ablation enhances the levels of γH2AX ubiquitylation. We also found that USP11 interacts with γH2AX both in vivo and in vitro. We found that 53BP1 and ubiquitin-conjugated proteins are misregulated to be retained longer and stronger at DSB sites after knockdown of USP11. We further found that cells are hypersensitive to γ-irradiation after ablation of USP11. Together, our findings elucidate deeply and extensively the mechanism of RNF8/RNF168 and USP11 to maintain the proper status of ubiquitylation γH2AX to repair DSB.
Related Links	http://www.jbc.org/content/291/2/959.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4705413/pdf http://www.jbc.org/article/S0021925820362268/pdf
Ending Page	967
Page Count	9
Starting Page	959
ISSN	00219258
e-ISSN	1083351X
DOI	10.1074/jbc.m114.624478
Journal	Journal of Biological Chemistry
Issue Number	2
Volume Number	291
Language	English
Publisher	Elsevier BV
Publisher Date	2016-01-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Chemistry Cell Biology E3 Ubiquitin-protein Ligase Rnf8 (rnf8) H2a Histone Family, Member X (h2afx) Deubiquitylation (deubiquitination) Ubiquitin-dependent Protease Ubiquitylation (ubiquitination)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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