NDLI: The ubiquitin-specific protease USP8 deubiquitinates and stabilizes Cx43

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The ubiquitin-specific protease USP8 deubiquitinates and stabilizes Cx43

Content Provider	Scilit
Author	Sun, Jian Hu, Qianwen Peng, Hong Peng, Cheng Zhou, Liheng Lu, Jinsong Huang, Chuanxin
Copyright Year	2018
Description	Journal: Journal of Biological Chemistry Connexin-43 (Cx43, also known as GJA1) is the most ubiquitously expressed connexin isoform in mammalian tissues. It forms intercellular gap junction (GJ) channels, enabling adjacent cells to communicate both electrically and metabolically. Cx43 is a short-lived protein which can be quickly degraded by the ubiquitin-dependent proteasomal, endolysosomal, and autophagosomal pathways. Here, we report that the ubiquitin-specific peptidase 8 (USP8) interacts with and deubiquitinates Cx43. USP8 reduces both multiple monoubiquitination and polyubiquitination of Cx43 to prevent autophagy-mediated degradation. Consistently, knockdown of USP8 results in decreased Cx43 protein levels in cultured cells and suppresses intercellular communication, revealed by the dye transfer assay. In human breast cancer specimens, the expression levels of USP8 and Cx43 proteins are positively correlated. Taken together, these results identified USP8 as a crucial and bona fide deubiquitinating enzyme involved in autophagy-mediated degradation of Cx43.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5971442/pdf http://www.jbc.org/content/293/21/8275.full.pdf
Ending Page	8284
Page Count	10
Starting Page	8275
ISSN	00219258
e-ISSN	1083351X
DOI	10.1074/jbc.ra117.001315
Journal	Journal of Biological Chemistry
Issue Number	21
Volume Number	293
Language	English
Publisher	Elsevier BV
Publisher Date	2018-05-01
Access Restriction	Open
Subject Keyword	Journal: Journal of Biological Chemistry Cell Biology Deubiquitylation (deubiquitination) Ubiquitin Ligase Ubiquitylation (ubiquitination)
Content Type	Text
Resource Type	Article
Subject	Cell Biology Biochemistry Molecular Biology

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