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Highly sensitive monoclonal antibody-based immunoassays for boscalid analysis in strawberries
| Content Provider | Scilit |
|---|---|
| Author | Esteve-Turrillas, Francesc A. Mercader, Josep V. Agulló, Consuelo Abad-Somovilla, Antonio Abad-Fuentes, Antonio |
| Copyright Year | 2018 |
| Description | Journal: Food Chemistry Boscalid is an agrochemical recently developed for crop protection and the most significant member of the succinate dehydrogenase inhibitor group of fungicides. In this study, a collection of high-affinity monoclonal antibodies was generated to boscalid. By using a series of haptens with a linker at alternative tethering sites of the boscalid framework, specific antibodies were isolated as well as antibodies that also recognized the main boscalid metabolite. Two immunoassays were developed using different ELISA formats. Optimized assays displayed very high sensitivities (limits of detection were near 0.01µg/L). Trueness and precision for the determination of the target analyte in strawberry samples was evaluated. Moreover, immunoassay performance was validated with a reference chromatographic method using QuEChERS extracts of fruits from fungicide-treated crops. A monitoring study with strawberry samples from local markets was carried out by immunoassay, showing an occurrence of boscalid of 15% with a maximum residue concentration of 43µg/kg. |
| Related Links | https://digital.csic.es/bitstream/10261/179467/1/EsteveTurrillas_FoodChem-2018_DigitalCSIC.pdf |
| Ending Page | 9 |
| Page Count | 8 |
| Starting Page | 2 |
| ISSN | 03088146 |
| e-ISSN | 18737072 |
| DOI | 10.1016/j.foodchem.2017.06.013 |
| Journal | Food Chemistry |
| Volume Number | 267 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2018-11-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Food Chemistry Analytical Chemistry Boscalid (pubchem Cid: 213013) Competitive Elisa Metabolite M510f01 Monoclonal Antibodies |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Analytical Chemistry Food Science |
