NDLI: Multiclonal Invasion in Breast Tumors Identified by Topographic Single Cell Sequencing

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Multiclonal Invasion in Breast Tumors Identified by Topographic Single Cell Sequencing

Content Provider	Scilit
Author	Casasent, Anna K. Schalck, Aislyn Gao, Ruli Sei, Emi Long, Annalyssa Pangburn, William Casasent, Tod Meric-Bernstam, Funda Edgerton, Mary E. Navin, Nicholas E.
Copyright Year	2018
Description	Journal: Cell Summary Ductal carcinoma in situ (DCIS) is an early-stage breast cancer that infrequently progresses to invasive ductal carcinoma (IDC). Genomic evolution has been difficult to delineate during invasion due to intratumor heterogeneity and the low number of tumor cells in the ducts. To overcome these challenges, we developed Topographic Single Cell Sequencing (TSCS) to measure genomic copy number profiles of single tumor cells while preserving their spatial context in tissue sections. We applied TSCS to 1,293 single cells from 10 synchronous patients with both DCIS and IDC regions in addition to exome sequencing. Our data reveal a direct genomic lineage between in situ and invasive tumor subpopulations and further show that most mutations and copy number aberrations evolved within the ducts prior to invasion. These results support a multiclonal invasion model, in which one or more clones escape the ducts and migrate into the adjacent tissues to establish the invasive carcinomas.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5766405/pdf http://www.cell.com/article/S0092867417314496/pdf
ISSN	00928674
e-ISSN	10974172
DOI	10.1016/j.cell.2017.12.007
Journal	Cell
Issue Number	1-2
Volume Number	172
Language	English
Publisher	Elsevier BV
Publisher Date	2018-01-04
Access Restriction	Open
Subject Keyword	Journal: Cell Single-cell Sequencing Cancer Genomics Ductal Carcinoma in Situ Intratumor Heterogeneity Copy Number Evolution Clonal Evolution Breast Cancer Progression Genome Evolution Breast Cancer
Content Type	Text
Resource Type	Article
Subject	Biochemistry, Genetics and Molecular Biology

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