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The structural arrangement and dynamics of the heteromeric GluK2/GluK5 kainate receptor as determined by smFRET
| Content Provider | Scilit |
|---|---|
| Author | Litwin, Douglas B. Paudyal, Nabina Carrillo, Elisa Berka, Vladimir Jayaraman, Vasanthi |
| Copyright Year | 2019 |
| Description | Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes Kainate receptors, which are glutamate activated excitatory neurotransmitter receptors, predominantly exist as heteromers of GluK2 and GluK5 subunits in the mammalian central nervous system. There are currently no structures of the full-length heteromeric kainate receptors. Here, we have used single molecule FRET to determine the specific arrangement of the GluK2 and GluK5 subunits within the dimer of dimers configuration in a full-length receptor. Additionally, we have also studied the dynamics and conformational heterogeneity of the amino-terminal and agonist-binding domain interfaces associated with the resting and desensitized states of the full-length heteromeric kainate receptor using FRET-based methods. The smFRET data are compared to similar experiments performed on the homomeric kainate receptor to provide insight into the differences in conformational dynamics that distinguish the two functionally. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC6899175/pdf |
| Ending Page | 183001 |
| Page Count | 1 |
| Starting Page | 183001 |
| ISSN | 00052736 |
| DOI | 10.1016/j.bbamem.2019.05.023 |
| Journal | Biochimica et Biophysica Acta (BBA) - Biomembranes |
| Issue Number | 1 |
| Volume Number | 1862 |
| Language | English |
| Publisher | Elsevier BV |
| Publisher Date | 2019-06-11 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biochimica et Biophysica Acta (BBA) - Biomembranes Biochemistry and Molecular Biology Kainate Receptors Ligand Gated Ion Channels |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Biochemistry Biophysics |
