NDLI: Global analysis of SUMO chain function reveals multiple roles in chromatin regulation

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Global analysis of SUMO chain function reveals multiple roles in chromatin regulation

Content Provider	Scilit
Author	Srikumar, Tharan Lewicki, Megan C. Costanzo, Michael Tkach, Johnny M. van Bakel, Harm Tsui, Kyle Johnson, Erica S. Brown, Grant W. Andrews, Brenda J. Boone, Charles Giaever, Guri Nislow, Corey Raught, Brian
Copyright Year	2013
Description	Like ubiquitin, the small ubiquitin-related modifier (SUMO) proteins can form oligomeric “chains,” but the biological functions of these superstructures are not well understood. Here, we created mutant yeast strains unable to synthesize SUMO chains $(smt3^{allR}$) and subjected them to high-content microscopic screening, synthetic genetic array (SGA) analysis, and high-density transcript profiling to perform the first global analysis of SUMO chain function. This comprehensive assessment identified 144 proteins with altered localization or intensity in $smt3^{allR}$ cells, 149 synthetic genetic interactions, and 225 mRNA transcripts (primarily consisting of stress- and nutrient-response genes) that displayed a >1.5-fold increase in expression levels. This information-rich resource strongly implicates SUMO chains in the regulation of chromatin. Indeed, using several different approaches, we demonstrate that SUMO chains are required for the maintenance of normal higher-order chromatin structure and transcriptional repression of environmental stress response genes in budding yeast.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3613684/pdf http://jcb.rupress.org/content/jcb/201/1/145.full.pdf
Ending Page	163
Page Count	19
Starting Page	145
DOI	10.1083/jcb.201210019
Journal	Journal of Cell Biology
Issue Number	1
Volume Number	201
Language	English
Publisher	Rockefeller University Press
Publisher Date	2013-04-01
Access Restriction	Open
Subject Keyword	Cell Biology Function Proteins Stress Sumo Chains Regulation of Chromatin Ubiquitin Journal: Journal of Cell Biology (Vol- 201, Issue- 1)
Content Type	Text

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