NDLI: RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response

Please wait, while we are loading the content...

RNF111/Arkadia is a SUMO-targeted ubiquitin ligase that facilitates the DNA damage response

Content Provider	Scilit
Author	Poulsen, Sara L. Hansen, Rebecca K. Wagner, Sebastian A. Cuijk, Loes Van van Belle, Gijsbert Jacob Christiaan Streicher, Werner Wikström, Mats Choudhary, Chunaram Houtsmuller, Adriaan B. Marteijn, Jurgen Bekker-Jensen, Simon Mailand, Niels
Copyright Year	2013
Description	Protein modifications by ubiquitin and small ubiquitin-like modifier (SUMO) play key roles in cellular signaling pathways. SUMO-targeted ubiquitin ligases (STUbLs) directly couple these modifications by selectively recognizing SUMOylated target proteins through SUMO-interacting motifs (SIMs), promoting their K48-linked ubiquitylation and degradation. Only a single mammalian STUbL, RNF4, has been identified. We show that human RNF111/Arkadia is a new STUbL, which used three adjacent SIMs for specific recognition of poly-SUMO2/3 chains, and used Ubc13–Mms2 as a cognate E2 enzyme to promote nonproteolytic, K63-linked ubiquitylation of SUMOylated target proteins. We demonstrate that RNF111 promoted ubiquitylation of SUMOylated XPC (xeroderma pigmentosum C) protein, a central DNA damage recognition factor in nucleotide excision repair (NER) extensively regulated by ultraviolet (UV)-induced SUMOylation and ubiquitylation. Moreover, we show that RNF111 facilitated NER by regulating the recruitment of XPC to UV-damaged DNA. Our findings establish RNF111 as a new STUbL that directly links nonproteolytic ubiquitylation and SUMOylation in the DNA damage response.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3678163/pdf http://jcb.rupress.org/content/jcb/201/6/797.full.pdf
Ending Page	807
Page Count	11
Starting Page	797
DOI	10.1083/jcb.201212075
Journal	Journal of Cell Biology
Issue Number	6
Volume Number	201
Language	English
Publisher	Rockefeller University Press
Publisher Date	2013-06-10
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Damage Proteins Sims Ubiquitin Ligase Ner Ubiquitylation Sumoylated Journal: Journal of Cell Biology (Vol- 201, Issue- 6)
Content Type	Text

Central Library (ISO-9001:2015 Certified)
Indian Institute of Technology Kharagpur
Kharagpur, West Bengal, India | PIN - 721302

See location in the Map
03222 282435
Mail: support@ndl.gov.in

Sl.	Authority	Responsibilities	Communication Details
1	Ministry of Education (GoI), Department of Higher Education	Sanctioning Authority	https://www.education.gov.in/ict-initiatives
2	Indian Institute of Technology Kharagpur	Host Institute of the Project: The host institute of the project is responsible for providing infrastructure support and hosting the project	https://www.iitkgp.ac.in
3	National Digital Library of India Office, Indian Institute of Technology Kharagpur	The administrative and infrastructural headquarters of the project	Dr. B. Sutradhar bsutra@ndl.gov.in
4	Project PI / Joint PI	Principal Investigator and Joint Principal Investigators of the project	Dr. B. Sutradhar bsutra@ndl.gov.in Prof. Saswat Chakrabarti will be added soon
5	Website/Portal (Helpdesk)	Queries regarding NDLI and its services	support@ndl.gov.in
6	Contents and Copyright Issues	Queries related to content curation and copyright issues	content@ndl.gov.in
7	National Digital Library of India Club (NDLI Club)	Queries related to NDLI Club formation, support, user awareness program, seminar/symposium, collaboration, social media, promotion, and outreach	clubsupport@ndl.gov.in
8	Digital Preservation Centre (DPC)	Assistance with digitizing and archiving copyright-free printed books	dpc@ndl.gov.in
9	IDR Setup or Support	Queries related to establishment and support of Institutional Digital Repository (IDR) and IDR workshops	idr@ndl.gov.in