NDLI: Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration

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Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration

Content Provider	Scilit
Author	Stramer, Brian Wood, William Galko, Michael J. Redd, Michael J. Jacinto, Antonio Parkhurst, Susan Martin, Paul
Copyright Year	2005
Description	Aa robust inflammatory response to tissue damage and infection is conserved across almost all animal phyla. Neutrophils and macrophages, or their equivalents, are drawn to the wound site where they engulf cell and matrix debris and release signals that direct components of the repair process. This orchestrated cell migration is clinically important, and yet, to date, leukocyte chemotaxis has largely been studied in vitro. Here, we describe a genetically tractable in vivo wound model of inflammation in the Drosophila melanogaster embryo that is amenable to cinemicroscopy. For the first time, we are able to examine the roles of Rho-family small GTPases during inflammation in vivo and show that Rac-mediated lamellae are essential for hemocyte motility and Rho signaling is necessary for cells to retract from sites of matrix– and cell–cell contacts. Cdc42 is necessary for maintaining cellular polarity and yet, despite in vitro evidence, is dispensable for sensing and crawling toward wound cues.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2171743/pdf http://jcb.rupress.org/content/168/4/567.full.pdf
Ending Page	573
Page Count	7
Starting Page	567
DOI	10.1083/jcb.200405120
Journal	Journal of Cell Biology
Issue Number	4
Volume Number	168
Language	English
Publisher	Rockefeller University Press
Publisher Date	2005-02-07
Access Restriction	Open
Subject Keyword	Cell Biology Developmental Biology Intercellular Junctions Cell Polarity Embryo Chemotaxis Pseudopodia Embryos Cell Migration Signal Transduction Lasers Journal: Journal of Cell Biology (Vol- 168, Issue- 4)
Content Type	Text

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