Live imaging of wound inflammation in Drosophila embryos reveals key roles for small GTPases during in vivo cell migration

Content Provider	Semantic Scholar
Author	Wood, Will Galko, Michael J. Jacinto, António Parkhurst, Susan M. Martin, Paul
Copyright Year	2005
Abstract	Aa robust inflammatory response to tissue damage and infection is conserved across almost all animal phyla. Neutrophils and macrophages, or their equivalents, are drawn to the wound site where they engulf cell and matrix debris and release signals that direct components of the repair process. This orchestrated cell migration is clinically important, and yet, to date, leukocyte chemotaxis has largely been studied in vitro. Here, we describe a genetically tractable in vivo wound model of inflammation in the Drosophila melanogaster embryo that is amenable to cinemicroscopy. For the first time, we are able to examine the roles of Rho-family small GTPases during inflammation in vivo and show that Rac-mediated lamellae are essential for hemocyte motility and Rho signaling is necessary for cells to retract from sites of matrix- and cell-cell contacts. Cdc42 is necessary for maintaining cellular polarity and yet, despite in vitro evidence, is dispensable for sensing and crawling toward wound cues.
Starting Page	567
Ending Page	573
Page Count	7
File Format	PDF HTM / HTML
PubMed reference number	15699212v1
Volume Number	168
Journal	The Journal of cell biology
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/168/4/567.full.pdf?with-ds=yes
Alternate Webpage(s)	http://research.fhcrc.org/content/dam/stripe/parkhurst/pdfs/stramer2005.pdf
Alternate Webpage(s)	http://jcb.rupress.org/content/jcb/168/4/567.full.pdf
Language	English
Access Restriction	Open
Subject Keyword	Amino Acids Chemotaxis Chemotaxis, Leukocyte Embryo Equivalent Weight Guanosine Triphosphate Phosphohydrolases Leukocytes Non-Small Cell Lung Carcinoma inflammatory response polarity
Content Type	Text
Resource Type	Article