NDLI: A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.

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A glycoprotein associated with the membrane fraction of human B but not T lymphocytes.

Content Provider	Scilit
Author	Fujita, Shigeru Litwin, Stephen D. Hartman, Neil
Copyright Year	1975
Description	Journal: The Journal of experimental medicine A method is described which employs differential centrifugation and sucrose density gradient centrifugation to isolate a membrane fraction from human lymphocytes. Membrane preparations from long-term human cultured B- and T-lymphoid lines, peripheral blood lymphocytes, tonsillar lymphocytes, and thymocytes were analyzed on 0.5% sodium dodecyl sulfate-7.5% polyacrylamide gels stained for protein and carbohydrate. The most important finding was a major glycoprotein of approximately 30,000 daltons associated with the membrane preparations from B lymphocytes. T-lymphocyte preparations did not contain readily detectable amounts of this membrane-associated component. The T-cell lymphoid line MOLT-4 was unique in that it had a narrow protein band at approximately 30,000 daltons which did not contain carbohydrate.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2190071/pdf http://jem.rupress.org/content/142/6/1416.full.pdf
ISSN	00221295
DOI	10.1084/jem.142.6.1416
Journal	The Journal of experimental medicine
Issue Number	6
Volume Number	142
Language	English
Publisher	Rockefeller University Press
Publisher Date	1975-12-01
Access Restriction	Open
Subject Keyword	Journal: The Journal of experimental medicine Analytical Chemistry Cell Line Immunoglobulins Glycoproteins Glycoprotein
Content Type	Text
Resource Type	Article
Subject	Physiology

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