NDLI: The SH3 domain of the Saccharomyces cerevisiae peroxisomal membrane protein Pex13p functions as a docking site for Pex5p, a mobile receptor for the import PTS1-containing proteins.

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The SH3 domain of the Saccharomyces cerevisiae peroxisomal membrane protein Pex13p functions as a docking site for Pex5p, a mobile receptor for the import PTS1-containing proteins.

Content Provider	Scilit
Author	Elgersma, Ype Kwast, L. Klein, A. Voorn-Brouwer, T. Berg, M. Van Den Metzig, B. America, Antoine Tabak, H. F. Distel, B.
Copyright Year	1996
Description	We identified a Saccharomyces cerevisiae peroxisomal membrane protein, Pex13p, that is essential for protein import. A point mutation in the COOH-terminal Src homology 3 (SH3) domain of Pex13p inactivated the protein but did not affect its membrane targeting. A two-hybrid screen with the SH3 domain of Pex13p identified Pex5p, a receptor for proteins with a type I peroxisomal targeting signal (PTS1), as its ligand. Pex13p SH3 interacted specifically with Pex5p in vitro. We determined, furthermore, that Pex5p was mainly present in the cytosol and only a small fraction was associated with peroxisomes. We therefore propose that Pex13p is a component of the peroxisomal protein import machinery onto which the mobile Pex5p receptor docks for the delivery of the selected PTS1 protein.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2121018/pdf
DOI	10.1083/jcb.135.1.97
Journal	Journal of Cell Biology
Issue Number	1
Volume Number	135
Language	English
Publisher	Rockefeller University Press
Publisher Date	1996-10-01
Access Restriction	Open
Subject Keyword	Biochemistry and Molecular Biology Protein Docking Domain Functions Saccharomyces Sh3 Peroxisomal Pts1 Pex5p Pex13p Journal: Journal of Cell Biology (Vol- 135, Issue- 1)
Content Type	Text

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