NDLI: Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development

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Endomucin, a CD34-like sialomucin, marks hematopoietic stem cells throughout development

Content Provider	Scilit
Author	Matsubara, Azusa Iwama, Atsushi Yamazaki, Satoshi Furuta, Chie Hirasawa, Ryutaro Morita, Yohei Osawa, Mitsujiro Motohashi, Tsutomu Eto, Koji Ema, Hideo Kitamura, Toshio Vestweber, Dietmar Nakauchi, Hiromitsu
Copyright Year	2005
Description	To detect as yet unidentified cell-surface molecules specific to hematopoietic stem cells (HSCs), a modified signal sequence trap was successfully applied to mouse bone marrow (BM) CD34−c-Kit+Sca-1+Lin− (CD34−KSL) HSCs. One of the identified molecules, Endomucin, is an endothelial sialomucin closely related to CD34. High-level expression of Endomucin was confined to the BM KSL HSCs and progenitor cells, and, importantly, long-term repopulating (LTR)–HSCs were exclusively present in the Endomucin+CD34−KSL population. Notably, in the yolk sac, Endomucin expression separated multipotential hematopoietic cells from committed erythroid progenitors in the cell fraction positive for CD41, an early embryonic hematopoietic marker. Furthermore, developing HSCs in the intraembryonic aorta-gonad-mesonephros (AGM) region were highly enriched in the CD45−CD41+Endomucin+ fraction at day 10.5 of gestation (E10.5) and in the CD45+CD41+Endomucin+ fraction at E11.5. Detailed analyses of these fractions uncovered drastic changes in their BM repopulating capacities as well as in vitro cytokine responsiveness within this narrow time frame. Our findings establish Endomucin as a novel cell-surface marker for LTR-HSCs throughout development and provide a powerful tool in understanding HSC ontogeny.
Related Links	http://jem.rupress.org/content/jem/202/11/1483.full.pdf https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2213340/pdf http://jem.rupress.org/content/202/11/1483.full.pdf
Ending Page	1492
Page Count	10
Starting Page	1483
DOI	10.1084/jem.20051325
Journal	The Journal of experimental medicine
Issue Number	11
Volume Number	202
Language	English
Publisher	Rockefeller University Press
Publisher Date	2005-11-28
Access Restriction	Open
Subject Keyword	Developmental Biology Research and Experimental Medicine Hematopoietic Bone Marrow Cd34 Progenitors Cd41 Cell Surface Cd45 Endomucin Ksl Journal: The Journal of experimental medicine (Vol- 202, Issue- 11)
Content Type	Text

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