NDLI: The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice

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The endothelial antigen ESAM marks primitive hematopoietic progenitors throughout life in mice

Content Provider	Scilit
Author	Yokota, Takafumi Oritani, Kenji Butz, Stefan Kokame, Koichi Kincade, Paul W. Miyata, Toshiyuki Vestweber, Dietmar Kanakura, Yuzuru
Copyright Year	2009
Description	Although recent advances have enabled hematopoietic stem cells (HSCs) to be enriched to near purity, more information about their characteristics will improve our understanding of their development and stage-related functions. Here, using microarray technology, we identified endothelial cell-selective adhesion molecule (ESAM) as a novel marker for murine HSCs in fetal liver. Esam was expressed at high levels within a Rag1− c-kitHi Sca1+ HSC-enriched fraction, but sharply down-regulated with activation of the Rag1 locus, a valid marker for the most primitive lymphoid progenitors in E14.5 liver. The HSC-enriched fraction could be subdivided into 2 on the basis of ESAM levels. Among endothelial antigens on hematopoietic progenitors, ESAM expression showed intimate correlation with HSC activity. The ESAMHi population was highly enriched for multipotent myeloid-erythroid progenitors and primitive progenitors with lymphopoietic activity, and exclusively reconstituted long-term lymphohematopoiesis in lethally irradiated recipients. Tie2+ c-kit+ lymphohematopoietic cells in the E9.5–10.5 aorta-gonad-mesonephros region also expressed high levels of ESAM. Furthermore, ESAM was detected on primitive hematopoietic progenitors in adult bone marrow. Interestingly, ESAM expression in the HSC-enriched fraction was up-regulated in aged mice. We conclude that ESAM marks HSC in murine fetal liver and will facilitate studies of hematopoiesis throughout life.
Related Links	http://www.bloodjournal.org/content/113/13/2914.full.pdf
Ending Page	2923
Page Count	10
Starting Page	2914
DOI	10.1182/blood-2008-07-167106
Journal	Blood
Issue Number	13
Volume Number	113
Language	English
Publisher	American Society of Hematology
Publisher Date	2009-03-26
Access Restriction	Open
Subject Keyword	Research and Experimental Medicine Antigen Bone Marrow Esam Enriched Primitive Functions Adult Hematopoietic Progenitors Journal: Blood (Vol- 123, Issue- 13)
Content Type	Text
Resource Type	Article

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