NDLI: GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITRO

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GENERATION OF CYTOTOXIC T LYMPHOCYTES IN VITRO

Content Provider	Scilit
Author	Cerottini, Jean-Charles Engers, Howard D. Donald, H. Robson Mac Brunner, K. Theodor
Copyright Year	1974
Description	Mouse cytotoxic T lymphocytes (CTL) were generated in mixed leukocyte cultures (MLC) using spleen cells as responding cells and irradiated allogeneic spleen cells as stimulating cells. Cytotoxicity was assessed by a quantitative 51Cr assay system and the relative frequency of CTL in individual cell populations was estimated from dose-response curves. Inclusion of 2-mercaptoethanol in the MLC medium resulted in a 20–40-fold increase in the relative number of CTL generated at the peak of the response. Under these culture conditions, cell-mediated cytotoxic activity was detectable in MLC populations as early as 48 h after the onset of the cultures. When spleen cells from mice immunized with allogeneic tumor cells 2–4 mo previously were cultured with irradiated spleen cells of the same alloantigenic specificity (MLC-Imm), it was found that the cell-mediated cytotoxic response was detectable earlier and reached higher levels than that observed in a primary MLC. At the peak of the response, MLC-Imm populations were observed to lyse up to 50% of the target cells within 3 h at a lymphocyte: target cell ratio of 0.3:1. Immunological and physical characterization of the effector cells generated in MLC-Imm indicated that they were medium to large-sized T lymphocytes. Altogether, these studies suggested the existence of an anamnestic cell-mediated cytotoxic response in MLC-Imm.
Related Links	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2139609/pdf http://jem.rupress.org/content/140/3/703.full.pdf
Ending Page	717
Page Count	15
Starting Page	703
DOI	10.1084/jem.140.3.703
Journal	The Journal of experimental medicine
Issue Number	3
Volume Number	140
Language	English
Publisher	Rockefeller University Press
Publisher Date	1974-09-01
Access Restriction	Open
Subject Keyword	Ctl Spleen Cells Mlc Imm Lymphocytes Cell Mediated Mediated Cytotoxic Journal: The Journal of experimental medicine (Vol- 140, Issue- 3)
Content Type	Text

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