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Antidiabetic, toxicological, and metabolomic profiling of aqueous extract of Cichorium intybus seeds
| Content Provider | Scilit |
|---|---|
| Author | Jain, Sk Khan, Washim Jetley, Sujata Ahmad, Sayeed Chandra, Kailash |
| Copyright Year | 2018 |
| Abstract | Background: Cichorium intybus has a wide range of therapeutic applications in Indian traditional systems of medicine, especially in metabolic disorders. Objective: To evaluate the toxicity profile and to investigate the antidiabetic, antihyperlipidemic, and antioxidative efficacy of C. intybus seeds in Wistar rats. Materials and Methods: The aqueous extract of seeds was prepared by decoction, and its quality control analysis was carried out by thin-layer chromatography and ultra-performance liquid chromatography-mass spectrometry (UPLC-MS) fingerprinting. Wistar rats were fed with high-fat diet for 5 weeks followed by a single dose of streptozotocin intraperitoneally to induce diabetes. The protective group of rats was given aqueous extract during and after the induction of type 2 diabetes mellitus. Further, repeated dose 28-day (subacute) and repeated dose 90-day (chronic) toxicity studies were conducted as per the OECD guidelines. Results: A total of 18 metabolites have been tentatively identified by UPLC-MS profiling in aqueous extract of C. intybus seeds. No significant changes in mortality and biochemical parameters have been observed during toxicity studies. Moreover, administration of the extract to a protective group of diabetic rats attenuated serum glucose and triglyceride levels by 52.7% and 65.3%, respectively, supported by similar results for parameters related to insulin resistance and oxidative stress. The beneficial effect of extract has also been confirmed through in silico screening. Conclusion: C. intybus can be used as a natural dietary supplement for the prevention and management of diabetes and can be explored to develop a potent phytopharmaceutical for diabetes. Abbreviations used: AECIS: Aqueous extract of Cichorium intybus seeds; HOMA-IR: Homeostatic model assessment of insulin resistance; OGTT: Oral glucose tolerance test; TLC: Thin-layer chromatography; UPLC-MS: Ultra-performance liquid chromatography-mass spectrometry. |
| Related Links | http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=57;spage=377;epage=383;aulast=Chandra;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/pm.pm_583_17 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 57 |
| Volume Number | 14 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2018-01-01 |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Chicory Docking Homeostatic Model Assessment of Insulin Resistance Thin-layer Chromatography Ultra-performance Liquid Chromatography-mass Spectrometry Pharmacognosy Magazine, Volume 14, Issue 57 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
