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In vitro anticholinesterase and neurotoxicity activities of Ocotea aciphylla fractions
| Content Provider | Scilit |
|---|---|
| Author | Botura, Mariana Borges Oliveira, Joana Da Luz Costa, Silvia Lima Carneiro, Monique Marylin A. de A. Reis, Isabella Mary Alves Silva, Alessandra Bispo Da Branco, Alexsandro Rodrigo, Souza Conceição |
| Copyright Year | 2018 |
| Abstract | Background: Ocotea species are known to produce secondary metabolites with a range of biological activities. This study aimed to evaluate the in vitro acetylcholinesterase (AChE) inhibition and neurotoxicity activities of the Ocotea aciphylla leaves. Materials and Methods: The in vitro anticholinesterase effect of crude extracts of O. aciphylla was investigated by means of spectrophotometric microplate assay. The most active extract, aqueous extract (AQE), was fractionated using column chromatography with silica gel as stationary phase to furnish several fractions that were also evaluated for the anticholinesterase effect. The neurotoxicity activity of AQE and active fraction (F9) was investigated in rat adrenal medulla pheochromocytoma strain cultures by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide test. The chemical characterization of the most active fraction was performed through high-performance liquid chromatography coupled with mass spectrometry multistage (HPLC-MS/MS). Results: Ethanolic extract (EE) and AQE exhibited significant inhibitory effects of the activity of AChE, with inhibitory concentration $(IC_{50}$) of 443.7 and 412.8 μg/mL, respectively. Among the fractions, F9 was more effective AChE inhibition with $IC_{50}$of 286.2 μg/mL. In the neurocytotoxicity assays, only the F9, at the highest concentration (500 μg/mL), induced a significant reduction in cell viability. HPLC-MS/MS analysis of the active fraction enabled the characterization of the natural compounds, i.e., procyanidin B-type dimer, propelargonidin dimer, catechin, and methoxy-luteolin-deoxyhexose-hexose. Conclusion: The leaves of O. aciphylla showed in vitro anticholinesterase activity and low neurotoxicity, and these effects might be related to the presence of phenolic compounds. Abbreviation used: AChE: acetylcholinesterase; ACTI: Acetylthiocholine iodide; AD: Alzheimer's disease; AQE: Aqueous extract; BSA: Bovine serum albumin; DMSO: Dimethyl sulfoxide; DTNB: 5,5'-dithiobis (2-nitrobenzoic acid); EAE: Ethyl acetate extract; EE: Ethanolic extract; EtOAc: Ethyl acetate; HE: Hexane extract; Hex: Hexane; HPLC-MS/MS: High-performance liquid chromatography coupled with mass spectrometry multistage; MeOH: Methanol; MTT: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide; PC-12: Rat adrenal medulla pheochromocytoma strain; RPMI: Roswell park memorial institute medium. |
| Related Links | http://www.phcog.com/article.asp?issn=0973-1296;year=2018;volume=14;issue=57;spage=448;epage=452;aulast=de;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/pm.pm_366_17 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 57 |
| Volume Number | 14 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2018-01-01 |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Acetylcholinesterase Cytotoxicity High-performance Liquid Chromatography-mass Spectrometry Ocotea Aciphylla Phenolic Compounds Pharmacognosy Magazine, Volume 14, Issue 57 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
