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Melastoma malabathricum ethyl acetate fraction induces secondary necrosis in human breast and lung cancer cell lines
| Content Provider | Scilit |
|---|---|
| Author | David, Sheba R. Zulkipli, Ihsan Nazurah Zulhilmi, Nurul Ramizah Lee, Shirley Hf Rajabalaya, Rajan Chee, Lim Y. Idris, Adi Majid, Mohamed |
| Copyright Year | 2017 |
| Abstract | Background: Melastoma malabathricum (MM) is a traditional plant used in the Borneo region. The cytotoxic effects of methanol extracts from MM leaves have been reported in a number of human cancer cell lines. However, the mode of cell death by MM has not been investigated. Objective: We investigated the cytotoxic effects of MM in both human breast and lung cancer cell lines, MCF-7 and A549, respectively, and defined the mode of cell death. Materials and Methods: Cell viability was measured using the 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay. Annexin-V/propidium iodide (PI) and terminal deoxynucleotidyl transferase dUTP nick-end labeling (TUNEL) staining was done to determine the mode of cell death. Results: The MTT assay revealed that MM extract had an IC50 of >400 μ g/ml on both cell lines at 24 h posttreatment. Flow cytometric and fluorescence microscopy analysis of Annexin-V/PI stained MM-treated cells revealed that the majority of the cells underwent secondary necrosis/late apoptosis. TUNEL assay showed that little to no DNA nicks were present in MM-treated cells, suggesting that cells have undergone secondary necrosis, not late apoptosis, at that time point. Conclusion: MCF-7 and A549 cells undergoes secondary necrosis 24 h post-treatment with MM extract. MM leaf extract could be a potential source for a novel anti-tumor agent for cancer therapy. Abbreviations used: DMSO: Dimethyl sulfoxide; MM: Melastoma malabathricum; MTT: 3-(4-, 5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide; PI: Propidium iodide; TUNEL: Terminal deoxynucleotidyl transferase dUTP nick-end labeling. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5669117/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2017;volume=13;issue=51;spage=688;epage=692;aulast=Idris;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/pm.pm_465_15 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 51 |
| Volume Number | 13 |
| Language | English |
| Publisher | Medknow |
| Publisher Date | 2017-01-01 |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Apoptosis Cancer Cytotoxic Flow Cytometry Melastoma Malabathricum Necrosis Pharmacognosy Magazine, Volume 13, Issue 51 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
