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Potency of Massoia bark in combating immunosuppressed-related infection
| Content Provider | Scilit |
|---|---|
| Author | Hertiani, Triana Pratiwi, Sylvia Utami Tunjung Yuswanto, Agustinus Permanasari, Prisci |
| Copyright Year | 2016 |
| Abstract | Background: As part of our search for new potential natural resources to eradicate infection, we have revealed the prominent potency of massoia bark (Massoia aromatica Becc, Lauraceae) in combating immunosuppressed-related infection. Materials and Methods: The extract was prepared by macerating the pulverized dried bark in ethanol 95%, followed by solvent evaporation. The oil was extracted from the dried bark by steam-hydrodistillation of which preparative thin-layer chromatography was performed on the oil to isolate the active constituent, C-10 massoia lactone (ML). Anti-biofilm assay against Candida albicans was conducted on polystyrene 96 wells microtiter plates, followed by a confocal laser scanning microscope observation to get three-dimensional profiles of the affected biofilms. Effects on the hyphae development inoculated on RPMI-1640 agar plates were observed for 7 days. Influences of samples on mice macrophage phagocytosis were examined by an in vitro technique. Samples concentration tested were in the range of 2.0–0.0625 mg/mL and done in triplicate. Results: Massoia bark extracts (oil and solid phase) and ML exhibited promising activities as anti-biofilm against C. albicans at $IC_{50}$ 0.074% v/v, 271 μg/mL and 0.026 μg/mL, respectively. The ML did not inhibit the hyphae development at the concentration tested; however, the extracts showed inhibition at 62.5 μg/mL. Macrophage phagocytosis stimulation was correlated to the ML content. Conclusion: Massoia bark is potential to be developed as anti-infective in immunosuppressed condition of which the C10 ML $(C_{10}H_{16}O_{2}$) plays a major role in exerting activity. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4971958/pdf http://www.phcog.com/article.asp?issn=0973-1296;year=2016;volume=12;issue=46;spage=363;epage=370;aulast=Hertiani;type=2 |
| File Format | XHTML |
| ISSN | 09731296 |
| e-ISSN | 09764062 |
| DOI | 10.4103/0973-1296.185771 |
| Journal | Pharmacognosy Magazine |
| Issue Number | 46 |
| Volume Number | 12 |
| Language | English |
| Publisher | Medknow |
| Access Restriction | Open |
| Subject Keyword | Integrative and Complementary Medicine Biofilm Candida Albicans Macrophage Massoia Bark Massoia Lactone Pharmacognosy Magazine, Volume 12, Issue 46 |
| Content Type | Text |
| Resource Type | Article |
| Subject | Drug Discovery Pharmaceutical Science |
