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MiR-27a-3p Targeting GSK3β Promotes Triple-Negative Breast Cancer Proliferation and Migration Through Wnt/β-Catenin Pathway
| Content Provider | Scilit |
|---|---|
| Author | Wu, Ruizhen Zhao, Bingqing Ren, Xunxin Wu, Shiheng Liu, Mingzao Wang, Zipeng Liu, Wei |
| Copyright Year | 2020 |
| Description | Journal: Cancer Management and Research Background: Dysregulation of microRNAs (miRNAs) was found to play crucial roles in varieties of cancers, which affect tumor proliferation and migration. MiR-27a-3p has been identified as a tumor-related miRNA in liver cancer, lung cancer, and colorectal cancer. However, the function of miR-27a-3p in triple-negative breast cancer (TNBC) and its possible molecular mechanisms have still not been elucidated. Methods: QRT-PCR technique was used to detect the expression of miR-27a-3p in TNBC and normal breast cell lines or the effects of miR-27a-3p knockdown and overexpression in TNBC cell lines. Proliferation and migration were measured by CCK-8 method, colony formation, wound healing, and Transwell assays, respectively. Furthermore, we used a dual-luciferase reporter gene assay and Western blot analysis to identify GSK3β as a target of miR-27a-3p. Results: In this study, we found that miR-27a-3p expression was significantly elevated in TNBC cell lines. Database analysis suggested that TNBC patients with a high expression of miR-27a-3p have poorer overall survival possibilities. Overexpression of miR-27a-3p promotes TNBC cells proliferation, colony formation, and cell migration in vitro. Nevertheless, dual-luciferase reporter result showed that miR-27a-3p directly targeted the 3ʹ-UTR regions of GSK3β mRNA and negatively regulated its expression. Lastly, we demonstrated that miR-27a-3p inactivates Wnt/β-catenin signaling pathway via targeting GSK3β. Conclusion: These results indicate that expression of miR-27a-3p was highly expressed in TNBC and promoted tumor progression through attenuating GSK3β and may have a potential molecular-targeted strategy for TNBC therapy. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7386804/pdf https://www.dovepress.com/getfile.php?fileID=59991 |
| Ending Page | 6249 |
| Page Count | 9 |
| Starting Page | 6241 |
| ISSN | 11791322 |
| DOI | 10.2147/cmar.s255419 |
| Journal | Cancer Management and Research |
| Volume Number | ume 12 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cancer Management and Research Wnt/β-catenin Pathway Triple-negative Breast Cancer |
| Content Type | Text |
| Subject | Oncology |
