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Identification and characterization of an oleate hydratase-encoding gene fromBifidobacterium breve
| Content Provider | Scilit |
|---|---|
| Author | O'Connell, Kerry Joan Motherway, Mary O'connell Hennessey, Alan A. Brodhun, Florian Ross, R. Paul Feussner, Ivo Stanton, Catherine Fitzgerald, Gerald F. van Sinderen, Douwe |
| Copyright Year | 2013 |
| Description | Journal: Bioengineered Bifidobacteria are common commensals of the mammalian gastrointestinal tract. Previous studies have suggested that a bifidobacterial myosin cross reactive antigen (MCRA) protein plays a role in bacterial stress tolerance, while this protein has also been linked to the biosynthesis of conjugated linoleic acid (CLA) in bifidobacteria. In order to increase our understanding on the role of MCRA in bifidobacteria we created and analyzed an insertion mutant of the MCRA-encoding gene of B. breve NCFB 2258. Our results demonstrate that the MCRA protein of B. breve NCFB 2258 does not appear to play a role in CLA production, yet is an oleate hydratase, which contributes to bifidobacterial solvent stress protection. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/bioe.24159?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3813531/pdf |
| Ending Page | 321 |
| Page Count | 9 |
| Starting Page | 313 |
| ISSN | 21655979 |
| e-ISSN | 21655987 |
| DOI | 10.4161/bioe.24159 |
| Journal | Bioengineered |
| Issue Number | 5 |
| Volume Number | 4 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2013-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioengineered Biotechnology and Microbiology Bifidobacteria Conjugated Linoleic Acid Myosin Cross Reactive Antigen |
| Content Type | Text |
| Subject | Medicine Bioengineering Applied Microbiology and Biotechnology Biotechnology |
