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Comparison of Matrix Vesicles Derived from Normal and Osteoarthritic Human Articular Cartilage
| Content Provider | Scilit |
|---|---|
| Author | Derfus, B. A. Kurtin, S. M. Camacho, N. P. Kurup, I. Ryan, L. M. |
| Copyright Year | 1996 |
| Description | Journal: Connective tissue research Articular cartilage matrix vesicles (MVs) from normal human adult articular cartilage were examined for protein and enzyme content and biomineralizing capacity for comparison to MVs derived from human osteoarthritic (OA) cartilage. Femoral condylar and tibial plateau cartilage from each of 9 healthy donors ages 17–37y was enzymatically digested and serially ultracentrifuged to pellet MVs at 3 × 106 g-min. MV protein content, nucleoside triphosphate pyrophospho hydrolase (NTPPPH) specific activity (SA) and capacity for 45Ca precipitation were determined. MV precipitated mineral was examined using Fourier transform infrared spectroscopy (FTIR). Normal human cartilage yields 50% less MV pro-tein/g cartilage than OA cartilage (p <. 01). Normal human articular MVs possess 30–70x higher NTPPPH S A than cell-free digest. Mean NTPPPH S As of MVs derived from normal human cartilage are 3x higher than that of OA MVs (p <. 05) and normal MV NTPPPH SA appears to decrease with age (p <. 01). Normal human MVs support significantly higher calcium precipitation/mg MV protein in both ATP-dependent (p <. 01) and -independent (p =. 05) systems. The FTIR spectrum of MV mineral generated in the presence of ATP strongly resembles the standard spectrum for calcium pyrophosphate dihydrate (CPPD). The FTIR spectrum of MV mineral generated without ATP resembles that of carbonate-substituted apatite (AP). The fact that isolated MVs from normal cartilage generate pathologically relevant crystal phases in vitro implies that matrix integrity and substrate availability may be crucial factors in the control of pathologic biomineralization. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/03008209609029209 |
| Ending Page | 342 |
| Page Count | 6 |
| Starting Page | 337 |
| ISSN | 03008207 |
| e-ISSN | 16078438 |
| DOI | 10.3109/03008209609029209 |
| Journal | Connective tissue research |
| Issue Number | 1-4 |
| Volume Number | 35 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1996-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Connective tissue research Biomineralization Human Articular Derived From Normal |
| Content Type | Text |
| Subject | Orthopedics and Sports Medicine Cell Biology Molecular Biology Biochemistry Rheumatology |
