Loading...
Please wait, while we are loading the content...
Similar Documents
Specific applications of techniques
| Content Provider | Scilit |
|---|---|
| Author | Anderson, M. L. M. |
| Copyright Year | 2020 |
| Description | Filter hybridization is extensively used in mapping DNA. If a fragment of DNA is sufficiently long, it is likely to have recognition sites for one or more restriction enzymes and it is possible to work out their position. Mapping of cloned DNA involves digesting the DNA with restriction enzymes both singly and in combination. The digest is run on an agarose gel and from the size of bands, the position of enzyme sites can be deduced. If the DNA is blotted on to a membrane and hybridized with a probe, the position of the complementary sequence can be determined (Figure 15.1). Obtaining a map and locating the site of particular sequences is often necessary for characterizing a newly isolated piece of DNA. Book Name: Nucleic Acid Hybridization |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2009-0-21201-0&isbn=9781003076780&doi=10.1201/9781003076780-17&format=pdf |
| Ending Page | 204 |
| Page Count | 34 |
| Starting Page | 171 |
| DOI | 10.1201/9781003076780-17 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-07-31 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Nucleic Acid Hybridization Analytical Chemistry Sufficiently Fragment of Dna |
| Content Type | Text |
| Resource Type | Chapter |
