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Basic types of filter hybridization
| Content Provider | Scilit |
|---|---|
| Author | Anderson, M. L. M. |
| Copyright Year | 2020 |
| Description | In filter hybridization, single-stranded DNA or RNA is bound to an inert support in such a way that it is prevented from reacting with itself, but is available to form hybrids with single-stranded nucleic acid added in solution. The sequences in solution are known as the probe and are labeled with reporter molecules. After hybridization, nonreacting probe is removed by washing and hybrids on the filter are visualized by virtue of the reporter molecules. The filter is said to have been 'probed' for the sequence of interest, for example if the reacting species in solution is ribosomal RNA, the filter is said to have been probed for rRNA. Book Name: Nucleic Acid Hybridization |
| Related Links | https://content.taylorfrancis.com/books/download?dac=C2009-0-21201-0&isbn=9781003076780&doi=10.1201/9781003076780-8&format=pdf |
| Ending Page | 64 |
| Page Count | 8 |
| Starting Page | 57 |
| DOI | 10.1201/9781003076780-8 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-07-31 |
| Access Restriction | Open |
| Subject Keyword | Book Name: Nucleic Acid Hybridization Analytical Chemistry Virtue Washing Single Stranded Ribosomal Removed Filter Hybridization Nonreacting |
| Content Type | Text |
| Resource Type | Chapter |
