Loading...
Please wait, while we are loading the content...
Similar Documents
Metal lothionein Induction in Calcifying Growth Plate Cartilage Chondrocytes
| Content Provider | Scilit |
|---|---|
| Author | Litchfield, Theresa M. Sauer, Glenn R. |
| Copyright Year | 1996 |
| Description | Journal: Connective tissue research Metallothionein (MT) induction was studied in mineralizing cultures of chicken growth plate chondrocytes and quantitated using a Cd-saturation assay. In serum free media, MT induction was observed for Cd concentrations of 0.1 microM and greater and at Zn concentrations of 100 microM and greater. Supplementation of culture media with cysteine and/or methionine resulted in higher levels of MT induction and reduced toxicity during Cd exposure. Maximum MT induction appeared to coincide with the earliest culture stages during which important enzymes and matrix components are being synthesized. Of non-metal MT inducers tested, sodium butyrate caused a low level induction of MT while interleukin-1 had no effect on basal MT levels. 1,25-dihydroxyvitamin D increased MT induction. The steroid hormone dexamethasone caused a reduction in basal and induced MT levels. These findings suggest that MT regulation in growth plate chondrocytes differs significantly from what is known in other cell types and that this difference may be related to the mineralization of this tissue. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/03008209609029191 |
| Ending Page | 195 |
| Page Count | 7 |
| Starting Page | 189 |
| ISSN | 03008207 |
| e-ISSN | 16078438 |
| DOI | 10.3109/03008209609029191 |
| Journal | Connective tissue research |
| Issue Number | 1-4 |
| Volume Number | 35 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1996-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Connective tissue research Growth Plate Chondrocytes |
| Content Type | Text |
| Subject | Orthopedics and Sports Medicine Cell Biology Molecular Biology Biochemistry Rheumatology |
