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Salmon nasal cartilage proteoglycan enhances growth of normal human dermal fibroblast through Erk1/2 phosphorylation
| Content Provider | Scilit |
|---|---|
| Author | Sano, Masahiro Shang, Yi Nakane, Akio Saito, Tomoaki |
| Copyright Year | 2017 |
| Description | Journal: Bioscience, Biotechnology, and Biochemistry Proteoglycan (PG) is a heavily glycosylated protein, localized to cell surface and extracellular matrix, and has various functions. Recently, it has been gradually revealed that PG interacts with various growth factors and morphogens and regulates cellular functions. Although salmon nasal cartilage PG (Salmon-PG) increases proliferation of immortalized cells, its mechanism remains unclear. In this study, we confirmed the effect of Salmon-PG on normal human dermal fibroblast (NHDF) and investigated the mechanism of PG action on NHDF. Salmon-PG dose- and time-dependently increased NHDF proliferation. Receptor tyrosine kinase array revealed that Salmon-PG increased only Erk1/2 signaling. Erk1/2 phosphorylation was significantly increased by Salmon-PG in a time-(10 min) and dose-(400 or 800 μg/mL) dependent manner. MEK inhibitor suppressed the enhancement of NHDF proliferation by Salmon-PG. The overall findings indicate that Salmon-PG plays a role as a growth factor in NHDF via Erk1/2 activation, suggesting that Salmon-PG contributes to the maintenance of skin homeostasis. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/09168451.2017.1318695?needAccess=true |
| Ending Page | 1385 |
| Page Count | 7 |
| Starting Page | 1379 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/09168451.2017.1318695 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 7 |
| Volume Number | 81 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2017-05-02 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Biochemistry and Molecular Biology Proteoglycan Erk Phosphorylation Normal Human Dermal Fibroblast |
| Content Type | Text |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
