NDLI: Cloning and identification of saprolmycin biosynthetic gene cluster from Streptomyces sp. TK08046

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Cloning and identification of saprolmycin biosynthetic gene cluster from Streptomyces sp. TK08046

Content Provider	Scilit
Author	Kawasaki, Takashi Moriyama, Asako Nakagawa, Kazuya Imamura, Nobutaka
Copyright Year	2016
Description	Journal: Bioscience, Biotechnology, and Biochemistry Saprolmycins A–E are anti-Saprolegnia parasitica antibiotics. To identify the gene cluster for saprolmycin biosynthesis in Streptomyces sp. TK08046, polymerase chain reaction using aromatase and cyclase gene-specific primers was performed; the spr gene cluster, which codes for angucycline biosynthesis, was obtained from the strain. The cluster consists of 36 open reading frames, including minimal polyketide synthase, ketoreductase, aromatase, cyclase, oxygenase, and deoxy sugar biosynthetic genes, as defined by homology to the corresponding genes of the urdamycin, Sch-47554, and grincamycin biosynthetic gene clusters in Streptomyces fradiae, Streptomyces sp. SCC-2136, and Streptomyces lusitanus, respectively. To establish the function of the gene cluster, an expression cosmid vector containing all 36 open reading frames was introduced into Streptomyces lividans TK23. The transformant was confirmed to express the biosynthetic genes and produce saprolmycins by liquid chromatography–mass spectrometry analysis of the extract.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/09168451.2016.1196574?needAccess=true https://academic.oup.com/bbb/article-pdf/80/11/2144/36832913/bbb2144.pdf
Ending Page	2150
Page Count	7
Starting Page	2144
ISSN	09168451
e-ISSN	13476947
DOI	10.1080/09168451.2016.1196574
Journal	Bioscience, Biotechnology, and Biochemistry
Issue Number	11
Volume Number	80
Language	English
Publisher	Informa UK Limited
Publisher Date	2016-11-01
Access Restriction	Open
Subject Keyword	Journal: Bioscience, Biotechnology, and Biochemistry Biotechnology and Microbiology Biosynthesis Gene
Content Type	Text
Subject	Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology

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