NDLI: In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2

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In planta functions of cytochrome P450 monooxygenase genes in the phytocassane biosynthetic gene cluster on rice chromosome 2

Content Provider	Scilit
Author	Ye, Zhongfeng Yamazaki, Kohei Minoda, Hiromi Miyamoto, Koji Miyazaki, Sho Kawaide, Hiroshi Yajima, Arata Nojiri, Hideaki Yamane, Hisakazu Okada, Kazunori
Copyright Year	2018
Description	In response to environmental stressors such as blast fungal infections, rice produces phytoalexins, an antimicrobial diterpenoid compound. Together with momilactones, phytocassanes are among the major diterpenoid phytoalexins. The biosynthetic genes of diterpenoid phytoalexin are organized on the chromosome in functional gene clusters, comprising diterpene cyclase, dehydrogenase, and cytochrome P450 monooxygenase genes. Their functions have been studied extensively using in vitro enzyme assay systems. Specifically, P450 genes (CYP71Z6, Z7; CYP76M5, M6, M7, M8) on rice chromosome 2 have multifunctional activities associated with ent-copalyl diphosphate-related diterpene hydrocarbons, but the in planta contribution of these genes to diterpenoid phytoalexin production remains unknown. Here, we characterized cyp71z7 T-DNA mutant and CYP76M7/M8 RNAi lines to find that potential phytoalexin intermediates accumulated in these P450-suppressed rice plants. The results suggested that in planta, CYP71Z7 is responsible for C2-hydroxylation of phytocassanes and that CYP76M7/M8 is involved in C11α-hydroxylation of 3-hydroxy-cassadiene. Based on these results, we proposed potential routes of phytocassane biosynthesis in planta.
Related Links	https://www.tandfonline.com/doi/pdf/10.1080/09168451.2017.1398067?needAccess=true https://academic.oup.com/bbb/article-pdf/82/6/1021/36830746/bbb1021.pdf
Ending Page	1030
Page Count	10
Starting Page	1021
ISSN	09168451
e-ISSN	13476947
DOI	10.1080/09168451.2017.1398067
Journal	Bioscience, Biotechnology, and Biochemistry
Issue Number	6
Volume Number	82
Language	English
Publisher	Informa UK Limited
Publisher Date	2018-06-03
Access Restriction	Open
Subject Keyword	Journal: Bioscience, Biotechnology, and Biochemistry Biochemistry and Molecular Biology Cytochrome P450 Monooxygenase Diterpene Phytoalexins Gene Cluster Rice
Content Type	Text
Resource Type	Article
Subject	Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology

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