NDLI: In situ hybridization in paracoccidioidomycosis

Content Provider	Scilit
Author	Brito, T. De Sandhu, G. S. Kline, B. C. Aleff, R. A. Sandoval, M. P. Santos, R. T. Brandão, A. A. H. Lacaz, C. S.
Copyright Year	1999
Description	In situ hybridization (ISH) was performed using oral biopsies from patients with paracoccidioidomycosis and guinea pig testes inoculated with a culture of Paracoccidioides brasiliensis isolated from soil, employing both a 14 base-pair specific oligoprobe (ACT CCC CCG TGG TC) and its complementary sequence. When combining ISH with the Gridley stain which detects fungal cell walls, about 2-3% of the fungal cells present in the tissues were labelled. When the complementary probe was used, labelling was higher, reaching the 3% level.
Related Links	https://academic.oup.com/mmy/article-pdf/37/3/207/3434431/37-3-207.pdf
Ending Page	211
Page Count	5
Starting Page	207
ISSN	13693786
DOI	10.1080/j.1365-280x.1999.00222.x
Journal	Medical mycology
Issue Number	3
Volume Number	37
Language	English
Publisher	Informa UK Limited
Publisher Date	1999-01-01
Access Restriction	Open
Subject Keyword	Mycology in Situ Hybridization Paracoccidioidomycosis Testis Paracoccidioides Brasiliensis Biopsy Base Pairing Cell Wall Guinea
Content Type	Text
Resource Type	Article
Subject	Infectious Diseases Veterinary

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In situ hybridization in paracoccidioidomycosis