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Different fates of oocytes with DNA double-strand breaksin vitroandin vivo
| Content Provider | Scilit |
|---|---|
| Author | Lin, Fei Ma, Xue-Shan Wang, Zhen-Bo Wang, Zhong-Wei Luo, Yi-Bo Huang, Lin Jiang, Zong-Zhe Hu, Meng-Wen Schatten, Heide Sun, Qing-Yuan |
| Copyright Year | 2014 |
| Description | In female mice, despite the presence of slight DNA double-strand breaks (DSBs), fully grown oocytes are able to undergo meiosis resumption as indicated by germinal vesicle breakdown (GVBD); however, severe DNA DSBs do reduce and delay entry into M phase through activation of the DNA damage checkpoint. But little is known about the effect of severe DNA DSBs on the spindle assembly checkpoint (SAC) during oocyte maturation. We showed that nearly no first polar body (PB1) was extruded at 12 h of in vitro maturation (IVM) in severe DNA DSBs oocytes, and the limited number of oocytes with PB1 were actually at telophase. However, about 60% of the severe DNA DSBs oocytes which underwent GVBD at 2 h of IVM released a PB1 at 18 h of IVM and these oocytes did reach the second metaphase (MII) stage. Chromosome spread at MI and MII stages showed that chromosomes fragmented after GVBD in severe DNA DSBs oocytes. The delayed PB1 extrusion was due to the disrupted attachment of microtubules to kinetochores and activation of the SAC. At the same time, misaligned chromosome fragments became obvious at the first metaphase (MI) in severe DNA DSBs oocytes. These data implied that the inactivation of SAC during the metaphase-anaphase transition of first meiosis was independent of chromosome integrity. Next, we induced DNA DSBs in vivo, and found that the number of superovulated oocytes per mouse was significantly reduced; moreover, this treatment increased the percentage of apoptotic oocytes. These results suggest that DNA DSBs oocytes undergo apoptosis in vivo. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.4161/15384101.2015.945375?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4614914/pdf |
| Ending Page | 2680 |
| Page Count | 7 |
| Starting Page | 2674 |
| ISSN | 15384101 |
| e-ISSN | 15514005 |
| DOI | 10.4161/15384101.2015.945375 |
| Journal | Cell Cycle |
| Issue Number | 17 |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2014-09-02 |
| Access Restriction | Open |
| Subject Keyword | Biochemistry and Molecular Biology Ddr, Dna Damage Response Dna Double-strand Breaks Dsbs, Dna Double-strand Breaks Gvbd, Germinal Vesicle Breakdown Icl, Interstrand Crosslinks Ivm, in Vitro Maturation Mi, the First Metaphase Mii, the Second Metaphase Pb1, First Polar Body Pbe, Pb1 Extrusion Sac, Spindle Assembly Checkpoint Apoptosis Meiosis Oocyte Spindle Assembly Checkpoint |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Developmental Biology Medicine Molecular Biology |
