NDLI: TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation

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TGN38 is required for the metaphase I/anaphase I transition and asymmetric cell division during mouse oocyte meiotic maturation

Content Provider	Scilit
Author	Chen, Lei Ge, Zhao-Jia Wang, Zhen-Bo Sun, Tianyi Ouyang, Ying-Chun Sun, Qing-Yuan Sun, Ying-Pu
Copyright Year	2014
Description	The cellular functions of the trans-Golgi network protein TGN38 remain unknown. In this research, we studied the expression, localization and functions of TGN38 in the meiotic maturation of mouse oocytes. TGN38 was expressed at every stage of oocyte meiotic maturation and colocalized with γ-tubulin at metaphase I and metaphase II. The spindle microtubule disturbing agents nocodazole and taxol did not affect the colocalization of TGN38 and γ-tubulin. Depletion of TGN38 with specific siRNAs resulted in increased metaphase I arrest, accompanied with spindle assembly checkpoint activation and decreased first polar extrusion (PB1). In the oocytes that had extruded the PB1 after the depletion of TGN38, symmetric division occurred, leading to the production of 2 similarly sized cells. Moreover, the peripheral migration of metaphase I spindle and actin cap formation were impaired in TGN38-depleted oocytes. Our data suggest that TGN38 may regulate the metaphase I/anaphase I transition and asymmetric cell division in mouse oocytes.
Related Links	http://www.tandfonline.com/doi/pdf/10.4161/15384101.2015.945828?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4613595/pdf
Ending Page	2732
Page Count	10
Starting Page	2723
ISSN	15384101
e-ISSN	15514005
DOI	10.4161/15384101.2015.945828
Journal	Cell Cycle
Issue Number	17
Volume Number	13
Language	English
Publisher	Informa UK Limited
Publisher Date	2014-09-02
Access Restriction	Open
Subject Keyword	Journal: Cell Cycle Cell Biology Gv Germinal Vesicle Gvbd Germinal Vesicle Breakdown Golgi Apparatus Mi Metaphase of the First Meiosis Mii Metaphase of the Second Meiosis Mtocs Microtubule Organizing Centers Pb1 First Polar Body Extrusion Tgn Trans-golgi Network Tgn38 Asymmetric Cell Division Metaphase I/anaphase I Transition
Content Type	Text
Resource Type	Article
Subject	Cell Biology Developmental Biology Medicine Molecular Biology

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