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Multicellular Spheroids Formation on Hydrogel Enhances Osteogenic/Odontogenic Differentiation of Dental Pulp Stem Cells Under Magnetic Nanoparticles Induction
| Content Provider | Scilit |
|---|---|
| Author | Han, Xiao Tang, Shijia Wang, Lin Xu, Xueqin Yan, Ruhan Yan, Sen Guo, Zhaobin Hu, Ke Yu, Tingting Li, Mengping Li, Yuqin Zhang, Feimin Gu, Ning |
| Copyright Year | 2021 |
| Description | Introduction: Promotion odontogenic differentiation of dental pulp stem cells (DPSCs) is essential for dentin regeneration. Physical cellular microenvironment is of critical importance for stem cells differentiation and influences the function of other biological/chemical factors to differentiation. Methods: Based on adjusting the mechanical/interfacial properties of hydrogels, multicellular spheroids (MCSs) of DPSCs generated through self-organization. The spheroids were characterized by immunofluorescent staining and flow cytometry. Quantitative real-time polymerase chain reaction, alkaline phosphatase (ALP) activity assay, ALP staining and Alizarin Red S staining were performed to evaluate the osteogenic/odontogenic differentiation of DPSCs with or without magnetic iron oxide nanoparticles (IONPs) induction. Results: MCSs of DPSCs exhibited a significant upregulation of E-cadherin and N-cadherin and enriched CD146 positive subpopulation, along with a stronger osteogenic/odontogenic differentiation ability. Moreover, DPSCs spheroids showed more substantial osteogenic differentiation tendency than the classical two-dimensional cultured DPSCs under the stimulation of magnetic IONPs. Conclusion: Three-dimensional spheroids culture of DPSCs based on composite viscoelastic materials combined with mechanical/magnetic stimulation may provide a theoretical basis for the subsequent development of dentin or bone regeneration technology. |
| Related Links | https://www.dovepress.com/getfile.php?fileID=72087 |
| Ending Page | 5115 |
| Page Count | 15 |
| Starting Page | 5101 |
| e-ISSN | 11782013 |
| DOI | 10.2147/ijn.s318991 |
| Journal | International Journal of Nanomedicine |
| Volume Number | 16 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2021-07-01 |
| Access Restriction | Open |
| Subject Keyword | Dentistry and Oral Surgery Tunable Mechanical Properties Dental Pulp Stem Cells Magnetic Nanomaterials Osteogenic/odontogenic Differentiation Multicellular Spheroids |
| Content Type | Text |
| Resource Type | Article |
