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| Content Provider | Springer Nature : BioMed Central |
|---|---|
| Author | Lin, Wenzhen Gao, Li Jiang, Wenxin Niu, Chenguang Yuan, Keyong Hu, Xuchen Ma, Rui Huang, Zhengwei |
| Abstract | Background Extracellular matrix secretion and odontoblastic differentiation in human dental pulp stem cells (hDPSCs) are the cellular bases for reparative dentinogenesis. Osteomodulin (OMD) is a member of the small leucine-rich proteoglycan family distributed in the extracellular matrix but little is known about its role in osteo/odontogenic differentiation. The objective of this study was to investigate the role of OMD during osteo/odontoblastic differentiation of hDPSCs. Methods hDPSCs were selected using immune-magnetic beads and their capability of multi-differentiation was identified. OMD knockdown was achieved using short hairpin RNA (shRNA) lentivirus and was confirmed by western blot. Gene expression was measured by real-time qPCR and osteo/odontoblastic differentiation of hDPSCs was determined by alizarin red S staining. Results Compared with uninduced cells, the transcription of OMD was up-regulated by 35-fold at the late stage of osteo/odontogenic differentiation. shRNA-mediated gene silencing of OMD decreased the expression of odontoblastic genes, such as alkaline phosphatase (ALP), dentin matrix acidic phosphoprotein 1 (DMP1) and dentin sialophosphoprotein (DSPP). Besides, knockdown of OMD attenuated the mineralized nodules formation induced by osteo/odontogenic medium. Conclusions These results implied that OMD may play a pivotal role in modulating the osteo/odontoblastic differentiation of hDPSCs. |
| Related Links | https://bmcoralhealth.biomedcentral.com/counter/pdf/10.1186/s12903-018-0680-6.pdf |
| Ending Page | 8 |
| Page Count | 8 |
| Starting Page | 1 |
| File Format | HTM / HTML |
| ISSN | 14726831 |
| DOI | 10.1186/s12903-018-0680-6 |
| Journal | BMC Oral Health |
| Issue Number | 1 |
| Volume Number | 19 |
| Language | English |
| Publisher | BioMed Central |
| Publisher Date | 2019-01-22 |
| Access Restriction | Open |
| Subject Keyword | Dentistry Oral and Maxillofacial Surgery Human dental pulp stem cells Osteomodulin Osteo/odontoblastic differentiation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Dentistry |
| Journal Impact Factor | 2.6/2023 |
| 5-Year Journal Impact Factor | 3.2/2023 |
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