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circKRT7-miR-29a-3p-COL1A1 Axis Promotes Ovarian Cancer Cell Progression
| Content Provider | Scilit |
|---|---|
| Author | An, Qiang Liu, Ting Wang, Ming-Yang Yang, Yu-Jia Zhang, Zhen-Dong Lin, Zhen-Jiang Yang, Bing |
| Copyright Year | 2020 |
| Description | Background: Circular RNA (circRNA) has emerged as an important regulator in the progression of human diseases. However, the role of circRNAs in ovarian cancer remains largely unknown. Materials and Methods: DNA sequencing and PCR were used to identify the existence and expression of circKRT7. The targeting relationship between circKRT7/miR-29a-3p and miR-29a-3p/COL1A1 was verified by fluorescence reporter assay. In vitro, colony formation, transwell and wound healing assay were used to detect the effects of circKRT7 and miR-29a-3p on the proliferation, migration and invasion ability of ovarian cancer cells. In vivo, xenograft tumor model was performed to validate the role of circKRT7 and miR-29a-3p in tumor growth. Results: We found that circKRT7 can promote the proliferation and metastasis of ovarian cancer cells by absorbing miR-29a-3p, which leads to the up-regulation of COL1A1. In vitro, knock-down of circKRT7 can inhibit the migration and invasion of ovarian cancer cells. This effect of circKRT7 is achieved by adsorbing miR-29a-3p and subsequently COL1A1 release. In vivo experiments, the reduction of circKRT7 expression can also slow tumor growth, and this inhibition was partly counteracted after miR-29a-3p repression. Conclusion: Overall, circKRT7 promotes EMT-related cell progression by absorbing miR-29a-3p in ovarian cancer. This suggests the crucial role of circular RNA in the malignant evolution in cancer. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7490051/pdf https://www.dovepress.com/getfile.php?fileID=61292 |
| Ending Page | 8976 |
| Page Count | 14 |
| Starting Page | 8963 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s259033 |
| Journal | OncoTargets and therapy |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-09-01 |
| Access Restriction | Open |
| Subject Keyword | Oncology Womens Studies Ovarian Cancer Circular Rna |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
