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Biogenic zinc oxide nanoparticles-enhanced biosynthesis of lignans and neolignans in cell suspension cultures of Linum usitatissimum L
| Content Provider | Scilit |
|---|---|
| Author | Abbasi, Bilal Haider Zahir, Adnan Ahmad, Waqar Nadeem, Muhammad Giglioli-Guivarc’H, Nathalie Hano, Christophe |
| Copyright Year | 2019 |
| Description | Zinc oxide nanoparticles (NPs) have emerged as a novel elicitor for enhanced biosynthesis of secondary metabolites in in vitro plant cell cultures. The current study was aimed to explore elicitation abilities of ZnO-NPs for enhanced accumulation of lignans and neolignans in cell cultures of Linum usitatissimum. We optimized concentration of zinc oxide NPs before carrying out a full-fledged experiment. Subsequently, an optimum dose of 100 mg/l was introduced into the culture medium on day 0, days 0 and 15, and finally days 0 and 25. We observed that repeated elicitation stimulated various parameters and physiological responses in Linum usitatissimum cell cultures than one-time elicitation. Repeated elicitation of cell cultures on day 0 and 15 resulted in highest fresh weight (412.16 g/l) and lignans production (secoisolariciresinol diglucoside 284.12 mg/l: lariciresinol diglucoside 86.97 mg/l). Contrarily, repeated elicitation on day 0 and 25 resulted in highest DW (13.53 g/l), total phenolic production (537.44 mg/l), total flavonoid production (123.83 mg/l) and neolignans production (dehydrodiconiferyl alcohol glucoside 493.28 mg/l: guaiacylglycerol-β-coniferyl alcohol ether glucoside 307.69 mg/l). Enhancement in plant growth and secondary metabolites accumulation was several fold higher than controls. Furthermore, a linear relationship existed between total phenolic and flavonoid contents which in turn was correlated with higher antioxidant activities. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/21691401.2019.1596942?needAccess=true |
| Ending Page | 1373 |
| Page Count | 7 |
| Starting Page | 1367 |
| ISSN | 21691401 |
| e-ISSN | 2169141X |
| DOI | 10.1080/21691401.2019.1596942 |
| Journal | Artificial cells, nanomedicine, and biotechnology |
| Issue Number | 1 |
| Volume Number | 47 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2019-05-28 |
| Access Restriction | Open |
| Subject Keyword | Agronomy Paper and Wood Elicitation Zinc Oxide Nanoparticles Log Phase Lignans Neolignans Cell Culture |
| Content Type | Text |
| Resource Type | Article |
| Subject | Medicine Biomedical Engineering Biotechnology Pharmaceutical Science |
