NDLI: Purification and Characterization of a Boar Sperm Protein Interacting with Immunoglobulins from an Infertile Woman

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Purification and Characterization of a Boar Sperm Protein Interacting with Immunoglobulins from an Infertile Woman

Content Provider	Scilit
Author	Yoshida, H. Kamada, M. Koide, S. S.
Copyright Year	1992
Description	Immunoglobulin G (IgG) fraction was prepared from a serum obtained from an infertile woman (IS) that induced sperm agglutination of human and boar sperm. The antisperm antibodies interacted with a 50-kD boar sperm protein, determined by immunoblot. The 50-kD protein was extracted with deoxycholate (DOC) and purified by affinity chromatography on concanavalin A column, ion exchange chromatography on CM52 column, affinity chromatography on IS IgG-protein G-agarose, and by preparative electrophoresis with electroelution. The purified 50-kD protein migrated as a single homogeneous band when analyzed by sodium dodecyl sulfate polyacrylamide gel electrophoresis and by Western blot. The production of antibodies against specific sperm proteins may be a cause of immunological infertility.
Related Links	https://www.tandfonline.com/doi/pdf/10.3109/01485019208987677?needAccess=true
Ending Page	38
Page Count	6
Starting Page	33
ISSN	01485016
e-ISSN	15210375
DOI	10.3109/01485019208987677
Journal	Archives of andrology
Issue Number	1
Volume Number	28
Language	English
Publisher	Informa UK Limited
Publisher Date	1992-01-01
Access Restriction	Open
Subject Keyword	Journal: Archives of Andrology Biochemical Research Antibodies Sodium Chromatography Immunoblot Infertile Boar Sperm Sperm Protein
Content Type	Text
Resource Type	Article
Subject	Endocrinology

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