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The lncRNA LINC01194/miR-486-5p Axis Facilitates Malignancy in Non-Small Cell Lung Cancer via Regulating CDK4
| Content Provider | Scilit |
|---|---|
| Author | Xing, Zhiwei Zhang, Zhihua Gao, Yanjun Zhang, Xun Kong, Xianglong Zhang, Jianwu Bai, Hongzhong |
| Copyright Year | 2020 |
| Description | Background: This experimental design was based on lncRNA LINC01194 to explore the pathogenesis of NSCLC. Methods: RT-qPCR was used to detect the expression of lncRNA LINC01194 and miR-486-5p in NSCLC tissues and cell lines. CCK-8, colony formation, and transwell assays were used to examine the effects of lncRNA LINC01194 and miR-486-5p on NSCLC cell proliferation and migration invasiveness. For target gene prediction and screening, luciferase reporter assays were used to verify downstream target genes for lncRNA LINC01194 and miR-486-5p. The protein expression of CDK4 was detected using Western blotting. The tumor changes in mice were detected by in vivo experiments in nude mice. Results: LncRNA LINC01194 was highly expressed in NSCLC tissues and NSCLC lines (A549, H1299, H460 cells, H1975), and lncRNA LINC01194 significantly promoted cell proliferation and migration of NSCLC cells. MiR-486-5p was identified as a potential target for LINC01194, and miR-486-5p was expressed at a low level in NSCLC tissues and NSCLC lines (A549, H1299, H460 cells, H1975). CDK4 was identified as a potential target for miR-486-5p. LncRNA LINC01194 was able to inhibit miR-486-5p expression and upregulate the expression level of CDK4. Finally, the results of in vivo animal models confirmed that lncRNA LINC01194 promoted NSCLC progression by modulating the miR-486-5p/CDK4 axis. Conclusion: LncRNA LINC01194 promoted the progression of NSCLC by modulating the miR-486-5p/CDK4 axis. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167278/pdf https://www.dovepress.com/getfile.php?fileID=57401 |
| Ending Page | 3163 |
| Page Count | 13 |
| Starting Page | 3151 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s235037 |
| Journal | OncoTargets and therapy |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-04-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Oncology Cdk4 Invasion Lncrna Linc01194 Mir-486-5p Non-small Cell Lung Cancer Proliferation |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
