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LncRNA TTN-AS1 Promotes Progression of Non-Small Cell Lung Cancer via Regulating miR-491-5p/ZNF503 Axis
| Content Provider | Scilit |
|---|---|
| Author | Qi, Guanbin Li, Lei |
| Copyright Year | 2020 |
| Description | Background: Non-small cell lung cancer (NSCLC) is the most common type of lung cancer with high mortality worldwide. Long non-coding RNA (lncRNA) TTN antisense RNA1 (TTN-AS1) has been demonstrated to play a crucial role in a variety of cancers. This study was designed to investigate the function and molecular mechanism of lncRNA TTN-AS1 in NSCLC. Methods: The expression levels of TTN-AS1, miR-491-5p and zinc finger protein 503 (ZNF503) were examined by quantitative real-time polymerase chain reaction (qRT-PCR) or Western blot assay, respectively. Cell viability was detected by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Cell migration and invasion were assessed by transwell assay. Epithelial-to-mesenchymal transition (EMT)-related proteins were measured using Western blot assay. The relationship between TTN-AS1, miR-491-5p and ZNF503 was predicted by starBase2.0 and confirmed by dual-luciferase reporter assay. Xenograft tumor experiment was conducted to analyze the tumor growth in vivo. Results: The levels of TTN-AS1 and ZNF503 were up-regulated, while miR-491-5p expression was reduced in NSCLC tissues and cells. Knockdown of TTN-AS1 or ZNF503 suppressed cell proliferation, migration, invasion and EMT in NSCLC cells. Overexpression of ZNF503 reversed the effect of TTN-AS1 silencing on NSCLC progression. TTN-AS1 could modulate the expression of ZNF503 via sponging miR-491-5p. Furthermore, TTN-AS1 induced tumor growth in vivo. Conclusion: Inhibition of TTN-AS1 hindered cell proliferation, migration, invasion and EMT in NSCLC cells by modulating miR-491-5p/ZNF503 axis, providing a promising biomarker for NSCLC treatment. |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7335898/pdf https://www.dovepress.com/getfile.php?fileID=59327 |
| Ending Page | 6371 |
| Page Count | 11 |
| Starting Page | 6361 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s238890 |
| Journal | OncoTargets and therapy |
| Volume Number | 13 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2020-07-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Oncology Ttn-as1 Mir-491-5p Znf503 Progression Non-small Cell Lung Cancer |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
