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Partial Purification and Some Properties of Polyphenoloxidases from Sago Palm
| Content Provider | Scilit |
|---|---|
| Author | Okamoto, Akiko Imagawa, Hiroshi Arai, Yuji Ozawa, Tetsuo |
| Copyright Year | 1988 |
| Description | Two polyphenoloxidases (PPO I and PPO III, EC 1.10.3.1) were extracted and partially purified from sago palm pith by hydroxylapatite chromatography, DEAE-cellulose chromatography and gel filtration. Both purified isozymes gave a single activity band on polyacrylamide gel electrophoresis. The molecular weights of both enzymes were estimated to be 40,000. They had the same pH optima of 6.5 but different temperature optima, 35°C for PPO I and 45°C for PPO III. PPO I was stable at neutral to alkaline pH and PPO III at acidic pH. PPO III was somewhat more stable than PPO I when incubated at various temperatures for 15 min. PPO I and PPO III oxidized well DL-epicatechin and d-catechin, respectively. Both enzymes were strongly inhibited by KCN, Na-diethyldithiocarbamate and $NaHSO_{3}$. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.1080/00021369.1988.10869013?needAccess=true |
| Ending Page | 2222 |
| Page Count | 8 |
| Starting Page | 2215 |
| ISSN | 09168451 |
| e-ISSN | 13476947 |
| DOI | 10.1080/00021369.1988.10869013 |
| Journal | Bioscience, Biotechnology, and Biochemistry |
| Issue Number | 9 |
| Volume Number | 52 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1988-09-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Bioscience, Biotechnology, and Biochemistry Paper and Wood Chromatography Gel Palm Partial Purified Ppo I and Ppo Iii |
| Content Type | Text |
| Resource Type | Article |
| Subject | Organic Chemistry Medicine Analytical Chemistry Molecular Biology Biochemistry Applied Microbiology and Biotechnology Biotechnology |
