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A Preliminary Study on Cryopreservation Protocol Applicable to all Types ofDiospyros KakiThunb.
| Content Provider | Scilit |
|---|---|
| Author | Niu, Y. L. Zhang, Y. F. Zhang, Q. L. Luo, Z. R. |
| Copyright Year | 2010 |
| Description | Diospyros kaki Thunb. was divided into four types based on astringency in the fruit at harvest, presence of seed, and flesh colour: The factors that affected cryopreservation of D. kaki Thunb. were studied by modified droplet vitrification: preculture condition and $PVS_{2}$ treatment time. After cryopreservation, different concentration of Pluronic F-68 in the regenerating medium was also investigated. A suitable protocol was established: shoot tips of axillaries were precultured on MS (1/2) medium with 0.5mol/L sucrose and 10g/L Pluronic F-68 for 5 days, which were loaded in loading solution for 20 min at 20°C, and incubated in $PVS_{2}$ for 1.5 h at 0°C prior to direct plunge into liquid nitrogen. After rapid thawing at 40°C warm water bath and rinsed, the shoot tips were plated on the regeneration medium supplemented with 1.0mg/L TDZ, 0.6g/L PVP, 0.1% Pluronic F-68, 30g/L sucrose and 7g/L agar for one week in dark prior to exposure to normal conditions. Using this procedure in cryopreservation of D. kaki Thunb., the best regeneration rate was 80%, the average rate was 73% and the difference of four types was not significant at P=0.05. |
| Related Links | https://www.tandfonline.com/doi/pdf/10.2478/V10133-010-0052-0?needAccess=true |
| Ending Page | 1964 |
| Page Count | 5 |
| Starting Page | 1960 |
| ISSN | 13102818 |
| e-ISSN | 13143530 |
| DOI | 10.2478/v10133-010-0052-0 |
| Journal | Biotechnology & Biotechnological Equipment |
| Issue Number | 3 |
| Volume Number | 24 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2010-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Biotechnology & Biotechnological Equipment Infectious Diseases Cryopreservation Kaki Thunb Loaded Preculture Protocol Nitrogen Treatment Pluronic |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biotechnology |
