NDLI: A Preliminary Study on Cryopreservation Protocol Applicable to all Types of Diospyros Kaki Thunb.

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A Preliminary Study on Cryopreservation Protocol Applicable to all Types of Diospyros Kaki Thunb.

Content Provider	Taylor & Francis Online
Author	Luo, Z. R. Zhang, Q. L. Zhang, Y. F. Niu, Y. L.
Abstract	Diospyros kaki Thunb. was divided into four types based on astringency in the fruit at harvest, presence of seed, and flesh colour: The factors that affected cryopreservation of D. kaki Thunb. were studied by modified droplet vitrification: preculture condition and PVS2 treatment time. After cryopreservation, different concentration of Pluronic F-68 in the regenerating medium was also investigated. A suitable protocol was established: shoot tips of axillaries were precultured on MS (1/2) medium with 0.5mol/L sucrose and 10g/L Pluronic F-68 for 5 days, which were loaded in loading solution for 20 min at 20°C, and incubated in PVS2 for 1.5 h at 0°C prior to direct plunge into liquid nitrogen. After rapid thawing at 40°C warm water bath and rinsed, the shoot tips were plated on the regeneration medium supplemented with 1.0mg/L TDZ, 0.6g/L PVP, 0.1% Pluronic F-68, 30g/L sucrose and 7g/L agar for one week in dark prior to exposure to normal conditions. Using this procedure in cryopreservation of D. kaki Thunb., the best regeneration rate was 80%, the average rate was 73% and the difference of four types was not significant at P=0.05.
Starting Page	1960
Ending Page	1964
Page Count	5
File Format	PDF HTM / HTML
ISSN	13102818 13143530
DOI	10.2478/V10133-010-0052-0
Journal	Biotechnology & Biotechnological Equipment
Volume Number	24
Issue Number	3
Language	English
Publisher	Taylor & Francis
Publisher Date	2014-04-15
Access Restriction	Open
Subject Keyword	Diospyros kaki Thunb. Modified droplet vitrification Cryopreservation
Content Type	Text
Resource Type	Article
Subject	Biotechnology

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A Preliminary Study on Cryopreservation Protocol Applicable to all Types ofDiospyros KakiThunb.

Cryopreservation of persimmon (Diospyros kaki Thunb.) by vitrification of dormant shoot tips

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Development of SSR primers using ISSR–PCR in Diospyros kaki Thunb.

Effects of Photosynthate Transport and Water Flow to Young Fruit on Fruit Drop via Ethylene Synthesis in Persimmon (Diospyros kaki Thunb.)

A Preliminary Study on Cryopreservation Protocol Applicable to all Types of Diospyros Kaki Thunb.

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