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Spin Trapping of Free Radicals Formed During Peroxidation of Sarcolemmal Membranes (SLM)
| Content Provider | Scilit |
|---|---|
| Author | Arroyo, Carmen M. Mak, I. Tong Weglicki, William B. |
| Copyright Year | 1989 |
| Description | The generation of free radicals in a superoxide (O2-.) driven Fe+3 catalysed reactions with isolated myocytic sarcolemma using electron spin resonance was investigated. Incubation of highly purified canine myocytic sarcolemma in the presence of the spin trap, 2-methyl-2-nitrosopropane (MNP), followed by the addition of dihydroxyfurmarate (DHF) and Fe+3-ADP resulted in the generation and detection of radical adducts of this spin trap. Spin trapping of the alkyl radicals with 2-methyl-2-nitrosopropane led to the identification of methyl radical adduct following exposure to DHF/Fe+3-ADP. With sarcolemma and the alkyl nitroso compound, the only radical product trapped was the methyl radical formed by beta-scission of alkoxyl radical. The participation of hydroperoxide-derived radicals in this system verified that the decomposition of unsaturated hydroperoxy fatty acid does proceed via a free radical mechanism. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/10715768909073420 |
| Ending Page | 376 |
| Page Count | 8 |
| Starting Page | 369 |
| ISSN | 87550199 |
| DOI | 10.3109/10715768909073420 |
| Journal | Free radical research communications |
| Issue Number | 6 |
| Volume Number | 5 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1989-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Free Radical Research Communications Atomic, Molecular and Chemical Physics Free Radicals Sarcolemma Spin Trap Alkyl Adduct Radicals Formed Myocytic |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
