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Spin Trapping Study of Superoxide Production in Ferrous Ion Oxidation
| Content Provider | Scilit |
|---|---|
| Author | Kosaka, Hiroaki Shiga, Takeshi |
| Copyright Year | 1993 |
| Description | The oxidation of Fe2+ was investigated by electron paramagnetic resonance (EPR) spin trapping techniques with N-t-butyl-alpha-phenylnitrone (PBN) and dimethyl sulfoxide. Under pure oxygen, the spin adduct PBN/.OCH3 was rapidly generated by the addition of Fe2+ (0.2-1.2 mM) into phosphate buffer containing ethylenediaminetetraacetate (EDTA), dimethyl sulfoxide and PBN at pH 7.4, but it decayed. The decay process of PBN/.OCH3 consists of two components. The fast decay was dependent on Fe2+ concentration. Another was due to destruction of the spin adduct by superoxide anion (.O2-), because superoxide dismutase (SOD) markedly prevented the decay. Catalase decreased the yield of PBN/.OCH3. When Fe(3+)-EDTA and ascorbate were used instead of Fe(2+)-EDTA, similar phenomena were detected. These results demonstrate that Fe2+ reacts with O2 to generate .O2-, then H2O2, which produces .CH3 by the reaction with Fe2+ and dimethyl sulfoxide. The .OCH3 radical results from the reaction between .CH3 and O2. The adduct PBN/.OCH3 decays by the reaction with Fe2+ and .O2-. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/10715769309056s63 |
| Ending Page | s69 |
| Page Count | 7 |
| Starting Page | s63 |
| ISSN | 87550199 |
| DOI | 10.3109/10715769309056s63 |
| Journal | Free radical research communications |
| Issue Number | sup1 |
| Volume Number | 19 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1993-10-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Free Radical Research Communications Atomic, Molecular and Chemical Physics Fe2 Dimethyl Sulfoxide Spin Trapping Ch3 Adduct Edta |
| Content Type | Text |
| Resource Type | Article |
| Subject | Biochemistry |
