NDLI: IMART software for correction of motion artifacts in images collected in intravital microscopy

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IMART software for correction of motion artifacts in images collected in intravital microscopy

Content Provider	Scilit
Author	Dunn, Kenneth W. Lorenz, Kevin S. Salama, Paul Delp, Edward J.
Copyright Year	2014
Description	Intravital microscopy is a uniquely powerful tool, providing the ability to characterize cell and organ physiology in the natural context of the intact, living animal. With the recent development of high-resolution microscopy techniques such as confocal and multiphoton microscopy, intravital microscopy can now characterize structures at subcellular resolution and capture events at sub-second temporal resolution. However, realizing the potential for high resolution requires remarkable stability in the tissue. Whereas the rigid structure of the skull facilitates high-resolution imaging of the brain, organs of the viscera are free to move with respiration and heartbeat, requiring additional apparatus for immobilization. In our experience, these methods are variably effective, so that many studies are compromised by residual motion artifacts. Here we demonstrate the use of IMART, a software tool for removing motion artifacts from intravital microscopy images collected in time series or in three dimensions.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/intv.28210?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC4469201/pdf
ISSN	21659079
e-ISSN	21659087
DOI	10.4161/intv.28210
Journal	IntraVital
Issue Number	1
Volume Number	3
Language	English
Publisher	Informa UK Limited
Publisher Date	2014-01-01
Access Restriction	Open
Subject Keyword	Journal: Intravital Microscopic Research Fluorescence Microscopy Image Analysis Image Processing Image Registration in Vivo Imaging Intravital Microscopy Motion Artifact Multiphoton Microscopy
Content Type	Text
Resource Type	Article
Subject	Cell Biology Radiology, Nuclear Medicine and Imaging Histology Pathology and Forensic Medicine

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