NDLI: Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin

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Intravital imaging of a spheroid-based orthotopic model of melanoma in the mouse ear skin

Content Provider	Scilit
Author	Chan, Keefe T. Jones, Stephen W. Brighton, Hailey E. Bo, Tao Cochran, Shelly D. Sharpless, Norman E. Bear, James E.
Copyright Year	2013
Description	Multiphoton microscopy is a powerful tool that enables the visualization of fluorescently tagged tumor cells and their stromal interactions within tissues in vivo. We have developed an orthotopic model of implanting multicellular melanoma tumor spheroids into the dermis of the mouse ear skin without the requirement for invasive surgery. Here, we demonstrate the utility of this approach to observe the primary tumor, single cell actin dynamics, and tumor-associated vasculature. These methods can be broadly applied to investigate an array of biological questions regarding tumor cell behavior in vivo.
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/intv.25805?needAccess=true https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5523129/pdf
ISSN	21659079
e-ISSN	21659087
DOI	10.4161/intv.25805
Journal	IntraVital
Issue Number	2
Volume Number	2
Language	English
Publisher	Informa UK Limited
Publisher Date	2013-04-01
Access Restriction	Open
Subject Keyword	Journal: Intravital Dermis Ear Intravital Imaging Melanoma Multiphoton Microscopy Second Harmonic Generation
Content Type	Text
Resource Type	Article
Subject	Cell Biology Radiology, Nuclear Medicine and Imaging Histology Pathology and Forensic Medicine

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