NDLI: Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly

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Survivin knockdown combined with apoptin overexpression inhibits cell growth significantly

Content Provider	Scilit
Author	Liu, Qin Fu, Hanjiang Xing, Ruiyun Tie, Yi Zhu, Jie Sun, Zhixian Zheng, Xiaofei
Copyright Year	2008
Description	The initiation and progression of tumor is regulated by multiple genes. Survivin belongs to the inhibitor of apoptosis protein (IAP) family and is overexpressed in most types of human tumors. Apoptin, originally identified from chicken anemia virus (CAV), can specifically induce apoptosis of human tumor cells rather than normal cells. In this study, survivin expression was silenced by microRNA (miRNA)-mediated RNA interference (RNAi); meanwhile, the engineered miRNA vector was also designed to express apoptin gene. The apoptosis and cell growth were then examined by flow cytometry and MTT assay. The miRNA-mediated knockdown of survivin in combination with apoptin overexpression significantly induced apoptosis and inhibited cell growth. Importantly, the combined strategy was more effective on inducing apoptosis and inhibiting cell growth than either survivin downregulation or apoptin overexpression alone. Taken together, the combined strategy offers potential advantages in control of tumorigenesis, and thus deserves further research as a preferred approach in cancer gene therapy
Related Links	https://www.tandfonline.com/doi/pdf/10.4161/cbt.7.7.6100?needAccess=true
Ending Page	1060
Page Count	8
Starting Page	1053
ISSN	15384047
e-ISSN	15558576
DOI	10.4161/cbt.7.7.6100
Journal	Cancer Biology & Therapy
Issue Number	7
Volume Number	7
Language	English
Publisher	Informa UK Limited
Publisher Date	2008-07-01
Access Restriction	Open
Subject Keyword	Journal: Cancer Biology & Therapy Biochemistry and Molecular Biology Apoptosis Mtt Assay Gene Therapy Cell Growth Microrna Overexpression Rnai Survivin Combined with Apoptin
Content Type	Text
Resource Type	Article
Subject	Cancer Research Pharmacology Molecular Medicine Oncology

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