NDLI: MicroRNA‑330‑5p inhibits osteosarcoma cell growth and invasion by targeting the proto‑oncogene survivin.

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MicroRNA‑330‑5p inhibits osteosarcoma cell growth and invasion by targeting the proto‑oncogene survivin.

Content Provider	Europe PMC
Author	Wang, Hao Liu, Lei Fang, Shiyuan
Copyright Year	2019
Abstract	Increasing evidence has suggested the crucial role of the dysregulation of microRNAs (miRNAs) in osteosarcoma (OS) progression. MicroRNA (miR)-330-5p has been reported to exert tumor suppressive effects in various types of human cancer. However, the role of miR-330-5p in the development of OS and the underlying mechanism remain to be clarified. In the present study, miR-330-5p expression was found to be significantly decreased in OS tissues and cell lines. In addition, low miR-330-5p expression was highly correlated with the overall survival and clinical stage of OS. Overexpression of miR-330-5p inhibited the viability, migration and invasion, and promoted the apoptosis of OS cells, as well as induced cell cycle arrest at the G2/M phase. Subsequently, the proto-oncogene survivin was identified as a functional target of miR-330-5p, and this was validated using a luciferase reporter assay. It was also demonstrated that survivin expression was markedly increased in OS tissues, and that it was negatively correlated with the expression of miR-330-5p. Furthermore, overexpression of survivin significantly abrogated the tumor-suppressive effect induced by miR-330-5p on OS cells. In conclusion, these results revealed that the miR-330-5p/survivin axis has a significant tumor-suppressive effect on OS, and may serve as a diagnostic and therapeutic target for the treatment of OS.
ISSN	17912997
Journal	Molecular Medicine Reports
Volume Number	20
PubMed Central reference number	PMC6691255
Issue Number	3
PubMed reference number	31322187
e-ISSN	17913004
DOI	10.3892/mmr.2019.10447
Language	English
Publisher	D.A. Spandidos
Publisher Date	2019-07-01
Access Restriction	Open
Rights License	This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License, which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. Copyright: © Wang et al.
Subject Keyword	osteosarcoma microRNA-330-5p survivin
Content Type	Text
Resource Type	Article
Subject	Genetics Molecular Biology Biochemistry Cancer Research Molecular Medicine Oncology

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