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Culture and Characterization of Rat Middle-ear Epithelium
| Content Provider | Scilit |
|---|---|
| Author | van Blitterswijk, Clemens Ponec, Maria Muijen, Goos N. P. Van Wijsman, Marian C. Koerten, Henk K. Grote, Jan J. |
| Copyright Year | 1986 |
| Description | This study was performed to design a method for the culture of rat middle-ear epithelium and to apply the method to investigate the characteristics of this epithelium. Culture of explants of middle-ear epithelium in the presence of the epidermal growth factor was successful, whereas serial cultivation required 3T3 feeder cells in addition to the epidermal growth factor. Cultured middle-ear epithelium was studied by phase-contrast microscopy, transmission and scanning electron microscopy, and combined light and scanning electron microscopy (LM/SEM). These techniques showed similarity between the cultured and the natural middle-ear epithelium. Explants and outgrowths showed both flat polygonal and ciliated epithelial cells. In serial cultivation, however, only the first of these cell types was observed. Frequently, a single primary cilium was found on the cell surface. Transmission electron microscopy showed cross-linked envelopes whose formation was promoted by ionophore X537A. Cytokeratin was demonstrated by immunoblotting, immunofluorescence, and immunoperoxidase methods, using an anti-cytokeratin monoclonal antibody. The model described here permits study of the differentiation of middle-ear epithelium in vitro and may be of future value for the study of chronic middle-ear diseases. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/00016488609108632 |
| Ending Page | 466 |
| Page Count | 14 |
| Starting Page | 453 |
| ISSN | 00016489 |
| e-ISSN | 16512251 |
| DOI | 10.3109/00016488609108632 |
| Journal | Acta Oto-Laryngologica |
| Issue Number | 5 |
| Volume Number | 101 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1986-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Acta Oto-laryngologica Dentistry and Oral Surgery Pathology Culture Immunoblotting Cytokeratin Epidermal Middle Ear Epithelium |
| Content Type | Text |
| Resource Type | Article |
| Subject | Otorhinolaryngology |
