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LGR6 promotes the progression of gastric cancer through PI3K/AKT/mTOR pathway
| Content Provider | Scilit |
|---|---|
| Author | Ke, Jing Ma, Peng Chen, Jinpeng Qin, Jun Qian, Haixin |
| Copyright Year | 2018 |
| Description | LGR6 promotes the progression of gastric cancer through PI3K/AKT/mTOR pathway Jing Ke,1,2 Peng Ma,2 Jinpeng Chen,2 Jun Qin,2 Haixin Qian1 1Department of General Surgery, The First Affiliated Hospital of Soochow University, Suzhou, People's Republic of China; 2Department of General Surgery, The Affiliated Hospital of Nantong University, Nantong, People's Republic of China Background: In the present study, we aimed to investigate the role of LGR6 in the progression of gastric cancer (GC) and explore the intrinsic molecular mechanisms.Materials and methods: The lentiviral LGR6 shRNA (sh-LGR6) and lentiviral expression vector of LGR6 gene (OE-LGR6) were used to regulate the LGR6 expression. Furthermore, we performed in vitro experiments to observe whether PI3K/AKT/mTOR pathway was affected by LGR6 and assess the role of LGR6 in the proliferation, apoptosis, migration, and invasion of GC cells.Results: Our data showed that phosphorylated AKT and mTOR were downregulated by sh-LGR6 (P<0.05). The expressions of proapoptotic proteins Bax and Caspase-3 were upregulated by sh-LGR6 (P<0.05); the expression of antiapoptotic protein Bcl2 was downregulated by sh-LGR6 (P<0.001). Besides, the functional experiments proved that sh-LGR6 could promote the apoptosis of GC cells and inhibit the proliferation, invasion, and migration of GC cells (P<0.001). Compared with sh-LGR6, OE-LGR6 led to the opposite results.Conclusion: LGR6 is an antiapoptosis protein which controls the progression of GC through PI3K/AKT/mTOR pathway. More in vivo experiments and clinical trials are necessary to confirm the possibility of LGR6 in tumor therapy. Keywords: LGR6, pathway, tumor therapy, gastric cancer |
| Related Links | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC5973468/pdf https://www.dovepress.com/getfile.php?fileID=42215 |
| Ending Page | 3033 |
| Page Count | 9 |
| Starting Page | 3025 |
| ISSN | 11786930 |
| DOI | 10.2147/ott.s149303 |
| Journal | OncoTargets and therapy |
| Volume Number | 11 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2018-05-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Oncotargets and Therapy Oncology Lgr6 Pathway Tumor Therapy Gastric Cancer |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pharmacology (medical) Oncology |
