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Protease Inhibitor Eglin-C Affects Superoxide Anion Release but not Bacterial Killing by Human Polymorphonuclear Leukocytes
| Content Provider | Scilit |
|---|---|
| Author | Esposito, Anthony L. Clark, Carolyn A. Poirier, William J. Kephart, Phyllis A. |
| Copyright Year | 1988 |
| Description | In order to assess the influence of the protease inhibitor eglin-c on superoxide anion (O−2) release by human polymorphonuclear leukocytes (PMN), cells were secured from normal donors and stimulated with phorbol myristate acetate (PMA), opsonized zymosan, or n-formyl-methionyl-leucyl-phenylalanine (FMLP). In the presence of 100 fxg/ml eglin-c, the activation time was prolonged and the maximum linear rate of O−2 formation was depressed following stimulation with PMA; a concentration of 1000 Hg/ml eglin-c was required to produce a similar effect with opsonized zymosan. Eglin-c did not influence the activation time following stimulation with FMLP, but at 2000 Hg/ml, the protease inhibitor attenuated the rate of O−2 production in response to the chemotactic peptide. In the presence of cytochalasin B, the inhibitory effect of eglin-c on O−2 release following stimulation with FMLP became more pronounced. In spite of these alterations in O−2 formation, the protease inhibitor did not impair the bactericidal activity of PMN against Staphylococcus aureus. Therefore, we conclude that although eglin-c can disrupt the activation and the activity of the superoxide-generating system of human PMN, the effect is stimulus dependent and is not associated with an alteration in the microbicidal capacity of neutrophils against S. aureus. |
| Related Links | http://www.tandfonline.com/doi/pdf/10.3109/01902148809087841 |
| Ending Page | 756 |
| Page Count | 14 |
| Starting Page | 743 |
| ISSN | 01902148 |
| e-ISSN | 15210499 |
| DOI | 10.3109/01902148809087841 |
| Journal | Experimental lung research |
| Issue Number | 6 |
| Volume Number | 14 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 1988-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Experimental Lung Research Immunology Protease Inhibitor Superoxide Anion Inhibitor Eglin Human Polymorphonuclear Polymorphonuclear Leukocytes |
| Content Type | Text |
| Resource Type | Article |
| Subject | Pulmonary and Respiratory Medicine Molecular Biology Clinical Biochemistry |
