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Superparamagnetic iron oxide nanoparticle-labeled cells as an effective vehicle for tracking the GFP gene marker using magnetic resonance imaging
| Content Provider | Scilit |
|---|---|
| Author | Zhang, Zhuoli Mascheri, Nicole Dharmakumar, Rohan Fan, Zhaoyang Paunesku, Tatjana Woloschak, Gayle Li, Debiao |
| Copyright Year | 2009 |
| Description | Detection of a gene using magnetic resonance imaging (MRI) is hindered by the magnetic resonance (MR) targeting gene technique. Therefore it may be advantageous to image gene-expressing cells labeled with superparamagnetic iron oxide (SPIO) nanoparticles by MRI. The GFP-R3230Ac (GFP) cell line was incubated for 24 h using SPIO nanoparticles at a concentration of 20 µg Fe/mL. Cell samples were prepared for iron content analysis and cell function evaluation. The labeled cells were imaged using fluorescent microscopy and MRI. SPIO was used to label GFP cells effectively, with no effects on cell function and GFP expression. Iron-loaded GFP cells were successfully imaged with both fluorescent microscopy and T2*-weighted MRI. Prussian blue staining showed intracellular iron accumulation in the cells. All cells were labeled (100% labeling efficiency). The average iron content per cell was 4.75 ± 0.11 pg Fe/cell (P < 0.05 versus control). This study demonstrates that the GFP expression of cells is not altered by the SPIO labeling process. SPIO-labeled GFP cells can be visualized by MRI; therefore, GFP, a gene marker, was tracked indirectly with the SPIO-loaded cells using MRI. The technique holds promise for monitoring the temporal and spatial migration of cells with a gene marker and enhancing the understanding of cell- and gene-based therapeutic strategies. |
| Related Links | http://europepmc.org/articles/pmc2840716?pdf=render https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2840716/pdf http://www.celltherapyjournal.org/article/S1465324909702476/pdf |
| Ending Page | 51 |
| Page Count | 9 |
| Starting Page | 43 |
| ISSN | 14653249 |
| e-ISSN | 14772566 |
| DOI | 10.1080/14653240802420243 |
| Journal | Cytotherapy |
| Issue Number | 1 |
| Volume Number | 11 |
| Language | English |
| Publisher | Informa UK Limited |
| Publisher Date | 2009-01-01 |
| Access Restriction | Open |
| Subject Keyword | Journal: Cytotherapy Biomaterials Gfp-r3230ac (gfp) Cell Line Magnetic Resonance Imaging (mri) Optical Imaging Superparamagnetic Iron Oxide (spio) Labeling |
| Content Type | Text |
| Resource Type | Article |
| Subject | Cell Biology Immunology and Allergy Transplantation Cancer Research Immunology Genetics (clinical) Oncology |
